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Article
Open AccessNetworked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
This study evaluated whether cell sheets containing a network of lymphatic endothelial cells (LECs) promoted lymphangiogenesis after transplantation in vivo. Cell sheets with a LEC network were constructed by ...
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Article
Open AccessExternal pressure dynamics promote kidney viability and perfusate filtration during ex vivo kidney perfusion
Normothermic machine perfusion (NMP) has not yet been established as a technique for preserving organs for a day. A key contributing factor to the same is that the perfusing solutions cannot circulate continuo...
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Article
Open AccessBioartificial pulsatile cuffs fabricated from human induced pluripotent stem cell-derived cardiomyocytes using a pre-vascularization technique
There is great interest in the development of techniques to bioengineer pulsatile myocardial tissue as a next-generation regenerative therapy for severe heart failure. However, creation of thick myocardial gra...
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Protocol
Organ Bioluminescence Imaging Under Machine Perfusion Setting for Assessing Quality of Harvested Organ Preservation
Determination of organ viability over a period of time is a key technology in the process of organ preservation. However, a robust methodology to address this issue has not been established. Luciferase-express...
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Protocol
Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure
This chapter describes a method for creating tubular cardiac tissue in vitro. Thick cardiac tissue in a tubular configuration is prepared by stacking cell sheets stepwise on the inner wall of a segment of smal...
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Article
Continuous measurement of surface electrical potentials from transplanted cardiomyocyte tissue derived from human-induced pluripotent stem cells under physiological conditions in vivo
Recording the electrical potentials of bioengineered cardiac tissue after transplantation would help to monitor the maturation of the tissue and detect adverse events such as arrhythmia. However, a few studies...
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Chapter and Conference Paper
Reconstruction of a Vascular Bed with Perfusable Blood Vessels Using a Decellularized Porcine Small Intestine for Clinical Application
Engineering three-dimensional tissues in vitro for regenerative therapy is highly desired. Vascular beds with connectable arteries and veins play crucial roles in fabricating three-dimensional tissues in vitro an...
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Reference Work Entry In depth
Cell Sheets for Cardiac Tissue Engineering
Recent studies have reported that the injection of isolated cells can improve cardiac function in models of myocardial infarction. However, the loss of transplanted cells from the target site due to local hypo...
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Article
Engineering of functional cardiac tubes by stepwise transplantation of cardiac cell sheets onto intestinal mesentery
Implantable organ-like grafts made using tissue engineering techniques could potentially be used as circulatory assist devices in people with heart failure. The aims of this study were to engineer implantable,...
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Article
Open AccessCorrection to: Intermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
article Intermittent application
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Article
Open AccessIntermittent application of external positive pressure helps to preserve organ viability during ex vivo perfusion and culture
The perfusion of medium through blood vessels allows the preservation of donor organs and culture of bioengineered organs. However, tissue damage due to inadequate perfusion remains a problem. We evaluated whe...
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Living Reference Work Entry In depth
Cell Sheets for Cardiac Tissue Engineering
Recent studies have reported that the injection of isolated cells can improve cardiac function in models of myocardial infarction. However, the loss of transplanted cells from the target site due to local hypo...
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Article
Microfluidic vascular-bed devices for vascularized 3D tissue engineering: tissue engineering on a chip
In this report, we describe a microfluidic vascular-bed (micro-VB) device providing a platform for 3D tissue engineering with vascular network formation. The micro-VB device allows functional connections betwe...
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Article
Open AccessDinaciclib potently suppresses MCL-1 and selectively induces the cell death in human iPS cells without affecting the viability of cardiac tissue
Induced pluripotent stem (iPS) cells hold great potential for being a major source of cells for regenerative medicine. One major issue that hinders their advancement to clinic is the persistence of undifferent...
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Article
Open AccessTubular Cardiac Tissues Derived from Human Induced Pluripotent Stem Cells Generate Pulse Pressure In Vivo
Human induced pluripotent stem (iPS) cell-derived cardiac cells provide the possibility to fabricate cardiac tissues for transplantation. However, it remains unclear human bioengineered cardiac tissues functio...
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Article
Open AccessTRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues
The development of a suitable strategy for eliminating remaining undifferentiated cells is indispensable for the use of human-induced pluripotent stem (iPS) cell-derived cells in regenerative medicine. Here, w...
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Chapter
Cell Sheet Tissue Engineering for Heart Failure
In recent years, regenerative medicine using cells for treating tissue defects has been in the spotlight as a new treatment for severe heart failure. Direct injection of bone marrow-derived cells and isolated ...
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Protocol
Cell Sheet Technology for Cardiac Tissue Engineering
In this chapter, we describe the methods for the fabrication and transfer/transplantation of 3D tissues by using cell sheet technology for cardiac tissue regeneration. A temperature-responsive culture surface ...
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Article
Open AccessIn Vitro Engineering of Vascularized Tissue Surrogates
In vitro scaling up of bioengineered tissues is known to be limited by diffusion issues, specifically a lack of vasculature. Here, we report a new strategy for preserving cell viability in three-dimensional tissu...
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Article
Open AccessIn vitro fabrication of functional three-dimensional tissues with perfusable blood vessels
Artificially engineered tissues may have many therapeutic applications but complex tissues are hard to create in vitro. Here, Okano and colleagues report the production of functional cardiac tissue sheets with pe...
