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Article
Open AccessFusing the 3’UTR of seed storage protein genes leads to massive recombinant protein accumulation in seeds
The demand for recombinant proteins is rising dramatically, and effective production systems are currently being developed. The production of recombinant proteins in plants is a promising approach due to its l...
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Article
Open AccessPexophagy suppresses ROS-induced damage in leaf cells under high-intensity light
Although light is essential for photosynthesis, it has the potential to elevate intracellular levels of reactive oxygen species (ROS). Since high ROS levels are cytotoxic, plants must alleviate such damage. Ho...
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Article
Open AccessEndoplasmic reticulum-derived bodies enable a single-cell chemical defense in Brassicaceae plants
Brassicaceae plants have a dual-cell type of chemical defense against herbivory. Here, we show a novel single-cell defense involving endoplasmic reticulum (ER)-derived organelles (ER bodies) and the vacuoles. ...
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Article
Open AccessSoybean (Glycine max L.) triacylglycerol lipase GmSDP1 regulates the quality and quantity of seed oil
Seeds of soybean (Glycine max L.) are a major source of plant-derived oils. In the past, improvements have been made in the quantity and quality of seed oil. Triacylglycerols (TAGs) are the principal components o...
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Protocol
Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein–Protein Interactions in Plant Cells
The bimolecular fluorescence complementation (BiFC) assay is a powerful, flexible, and simple tool to study protein–protein interactions in living cells. To accelerate the production and assessment of BiFC con...
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Article
Erratum: Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis
Nature Plants 1, 15035 (2015); published online 30 March 2015; corrected 10 April 2015 In the version of the Supplementary Information file originally published figure captions were omitted and several charact...
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Article
Physical interaction between peroxisomes and chloroplasts elucidated by in situ laser analysis
Life on earth relies upon photosynthesis, which consumes carbon dioxide and generates oxygen and carbohydrates. Photosynthesis is sustained by a dynamic environment within the plant cell involving numerous org...
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Chapter and Conference Paper
The Role of Peroxisomes in Plant Reproductive Processes
Peroxisomes are ubiquitous organelles found in eukaryotic cells. These organelles perform important metabolic functions required for normal development. Peroxisomes play crucial roles in flowering plants, func...
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Article
A possible involvement of autophagy in amyloplast degradation in columella cells during hydrotropic response of Arabidopsis roots
Seedling roots display not only gravitropism but also hydrotropism, and the two tropisms interfere with one another. In Arabidopsis (Arabidopsis thaliana) roots, amyloplasts in columella cells are rapidly degrade...
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Article
Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice
Enzyme 12-oxophytodienoate (OPDA) reductase (EC1.3.1.42), which is involved in the biosynthesis of jasmonic acid (JA), catalyses the reduction of 10, 11-double bonds of OPDA to yield 3-oxo-2-(2′-pentenyl)-cycl...
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Article
The slow wound-response of γVPE is regulated by endogenous salicylic acid in Arabidopsis
Vacuolar processing enzyme (VPE) is a cysteine protease responsible for the maturation of various vacuolar proteins in higher plants. The Arabidopsis thaliana (L.) Heynh. γVPE gene, encoding a VPE homologue, is s...
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Chapter
Genetic Approaches to Understand Plant Peroxisomes
Peroxisomes in higher plant cells are known to differentiate in function depending on the cell type. Because of the functional differentiation, plant peroxisomes are sub-divided into at least three different c...
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Article
A leaf-peroxisomal protein, hydroxypyruvate reductase, is produced by light-regulated alternative splicing
Hydroxypyruvate reductase (HPR) is localized in leaf peroxisomes in plants, and it plays an important role in the glycolate pathway of photorespiration. In this laboratory, two highly homologous cDNAs for pump...
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Article
Transport of peroxisomal proteins synthesized as large precursors in plants
Plant peroxisomes contain at least four proteins, namely, citrate synthase, malate dehydrogenase, long-chain acyl-CoA oxidase, and 3-ketoacyl-CoA thiolase, which are synthesized as large precursors with an N-t...
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Article
Functional transformation of plant peroxisomes
Peroxisomes in higher plant cells are known to differentiate into at least three different classes, namely, glyoxysomes, leaf peroxisomes, and unspecialalized peroxisomes, dependending on the cell types. In ge...
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Article
Microbody proliferation and segregation cycle in the single-microbody alga Cyanidioschyzon merolae
The proliferation cycle of the microbody was studied in the primitive red alga Cyanidioschyzon merolae, which contains one microbody per cell. Cells were synchronized with a dark/light cycle, and the morphology ...
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Article
Microbody defective mutants of arabidopsis
In germinating fatty seedlings, microbodies are differentiated to leaf peroxisomes from glyoxysomes during greening, and then transformed to glyoxysomes from leaf peroxisomes during senescence. These transform...
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Article
cDNA cloning and differential gene expression of three catalases in pumpkin
Three cDNA clones (cat1, cat2, cat3) for catalase (EC 1.11.1.6) were isolated from a cDNA library of pumpkin (Cucurbita sp.) cotyledons. In northern blotting using the cDNA-specific probe, the cat1 mRNA levels...
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Article
cDNA cloning and expression of a gene for 3-ketoacyl-CoA thiolase in pumpkin cotyledons
A cDNA clone for 3-ketoacyl-CoA thiolase (EC 2.3.1.16) was isolated from a λgt11 cDNA library constructed from the poly(A)+ RNA of etiolated pumpkin cotyledons. The cDNA insert contained 1682 nucleotides and enco...
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Article
Pumpkin hydroxypyruvate reductases with and without a putative C-terminal signal for targeting to microbodies may be produced by alternative splicing
Two full-length cDNAs encoding hydroxypyruvate reductase were isolated from a cDNA library constructed with poly(A)+ RNA from pumpkin green cotyledons. One of the cDNAs, designated HPR1, encodes a polypeptide of ...
