28 Result(s)
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Shin-ya Miyagishima
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Article
Open AccessTaming the perils of photosynthesis by eukaryotes: constraints on endosymbiotic evolution in aquatic ecosystems
An ancestral eukaryote acquired photosynthesis by genetically integrating a cyanobacterial endosymbiont as the chloroplast. The chloroplast was then further integrated into many other eukaryotic lineages throu...
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Article
Open AccessA cotransformation system of the unicellular red alga Cyanidioschyzon merolae with blasticidin S deaminase and chloramphenicol acetyltransferase selectable markers
The unicellular red alga Cyanidioschyzon merolae exhibits a very simple cellular and genomic architecture. In addition, procedures for genetic modifications, such as gene targeting by homologous recombination and...
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Article
Open AccessGenome sequencing of the multicellular alga Astrephomene provides insights into convergent evolution of germ-soma differentiation
Germ-soma differentiation evolved independently in many eukaryotic lineages and contributed to complex multicellular organizations. However, the molecular genetic bases of such convergent evolution remain unre...
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Article
Cell size for commitment to cell division and number of successive cell divisions in cyanidialean red algae
Several eukaryotic cell lineages proliferate by multiple fission cell cycles, during which cells grow to manyfold of their original size, then undergo several rounds of cell division without intervening growth...
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Article
Open AccessEfficient open cultivation of cyanidialean red algae in acidified seawater
Microalgae possess high potential for producing pigments, antioxidants, and lipophilic compounds for industrial applications. However, their open pond cultures are often contaminated by other undesirable organ...
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Article
Open AccessResponses of unicellular predators to cope with the phototoxicity of photosynthetic prey
Feeding on unicellular photosynthetic organisms by unicellular eukaryotes is the base of the aquatic food chain and evolutionarily led to the establishment of photosynthetic endosymbionts/organelles. Photosynt...
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Article
Open AccessAnisogamy evolved with a reduced sex-determining region in volvocine green algae
Male and female gametes differing in size—anisogamy—emerged independently from isogamous ancestors in various eukaryotic lineages, although genetic bases of this emergence are still unknown. Volvocine green al...
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Article
Open AccessEvolution of cytokinesis-related protein localization during the emergence of multicellularity in volvocine green algae
The volvocine lineage, containing unicellular Chlamydomonas reinhardtii and differentiated multicellular Volvox carteri, is a powerful model for comparative studies aiming at understanding emergence of multicellu...
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Article
Open AccessRegulation of chloroplast and nucleomorph replication by the cell cycle in the cryptophyte Guillardia theta
The chloroplasts of cryptophytes arose through a secondary endosymbiotic event in which a red algal endosymbiont was integrated into a previously nonphotosynthetic eukaryote. The cryptophytes retain a remnant ...
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Article
Chloroplast division: A handshake across membranes
The chloroplast evolved from a symbiotic cyanobacterium and it still divides like one. Bacterial inner division machinery recruits the eukaryotic outer complex, which in turn condenses the inner ring. This rec...
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Article
Target of rapamycin (TOR) plays a critical role in triacylglycerol accumulation in microalgae
Most microalgae produce triacylglycerol (TAG) under stress conditions such as nitrogen depletion, but the underlying molecular mechanism remains unclear. In this study, we focused on the role of target of rapa...
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Article
Translation-independent circadian control of the cell cycle in a unicellular photosynthetic eukaryote
Circadian rhythms of cell division have been observed in several lineages of eukaryotes, especially photosynthetic unicellular eukaryotes. However, the mechanism underlying the circadian regulation of the cell...
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Article
Open AccessDipM is required for peptidoglycan hydrolysis during chloroplast division
Chloroplasts have evolved from a cyanobacterial endosymbiont and their continuity has been maintained over time by chloroplast division, a process which is performed by the constriction of a ring-like division...
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Article
Open AccessAnalysis of gamete membrane dynamics during double fertilization of Arabidopsis
Angiosperms have a unique sexual reproduction system called “double fertilization.” One sperm cell fertilizes the egg and another sperm cell fertilizes the central cell. To date, plant gamete membrane dynamics...
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Article
Open AccessThe YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria
Reminiscent of their free-living cyanobacterial ancestor, chloroplasts proliferate by division coupled with the partition of nucleoids (DNA-protein complexes). Division of the chloroplast envelope membrane is ...
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Chapter
The Chloroplast Division Machinery: Origin and Evolution
It is widely believed that chloroplasts and mitochondria arose from bacterial endosymbionts related to cyanobacteria and α-proteobacteria, respectively (reviewed in Reyes-Prieto et al., 2007; Fig. 1). Although...
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Article
Open AccessPlastid chaperonin proteins Cpn60α and Cpn60β are required for plastid division in Arabidopsis thaliana
Plastids arose from a free-living cyanobacterial endosymbiont and multiply by binary division as do cyanobacteria. Plastid division involves nucleus-encoded homologs of cyanobacterial division proteins such as...
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Chapter
The Mechanism of Plastid Division: The Structure and Origin of The Plastid Division Apparatus
Chloroplasts were derived from a free-living cyanobacterium which was engulfed by a primary non-photosynthetic eukaryotic host cell and subsequently evolved into a plastid. Plastids are never synthesized de novo ...
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Article
Origin and evolution of the chloroplast division machinery
Chloroplasts were originally established in eukaryotes by the endosymbiosis of a cyanobacterium; they then spread through diversification of the eukaryotic hosts and subsequent engulfment of eukaryotic algae b...
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Article
Open AccessGenome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D
Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support the lives of photosynthetic eukaryotes, including higher plants1,2. Here we report the 16,5...
