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  1. No Access

    Chapter

    Physiological Role of Ascorbic Acid Recycling Enzymes in Plants

    Ascorbic acid (AsA) is oxidized to monodehydroascorbate (MDHA), which dissociates to form dehydroascorbate (DHA) instead of detoxifying reactive oxygen species (ROS). MDHA and DHA are directly reduced to AsA b...

    Marina Suekawa, Yukichi Fujikawa… in Ascorbic Acid in Plant Growth, Development… (2017)

  2. No Access

    Chapter

    Regulation of Ascorbic Acid Biosynthesis in Plants

    Plants synthesize l-ascorbic acid (AsA) by the Smirnoff-Wheeler pathway, which is considered to be the principal de novo biosynthesis pathway for AsA. In addition, three alternative biosynthesis pathways have als...

    Marina Suekawa, Takayuki Kondo… in Ascorbic Acid in Plant Growth, Development… (2017)

  3. No Access

    Article

    Characterization of Secretory Phospholipase A2 with Phospholipase A1 Activity in Tobacco, Nicotiana tabacum (L.)

    A cDNA encoding protein with homology to plant secretory phospholipase A2 (sPLA2), denoted as Nt1 PLA2, was isolated from tobacco (Nicotiana tabacum). The cDNA encodes a mature protein of 118 amino acid residues ...

    Yukichi Fujikawa, Ritsuko Fujikawa, Noriaki Iijima, Muneharu Esaka in Lipids (2012)

  4. No Access

    Chapter

    Identification of Potential Gene Targets for the Improvement of Ascorbate Contents of Genetically Modified Plants

    Up to half of the ∼6.8 billion people living on planet earth live on less than $3 a day and also suffer from at least one micronutrient deficiency especially in Africa and Southeast Asia. Human lacks the capac...

    Adebanjo A. Badejo, Muneharu Esaka in Ascorbate-Glutathione Pathway and Stress T… (2010)

  5. No Access

    Article

    Molecular cloning, expression, and characterization of secretory phospholipase A2 in tobacco

    Phospholipase A2 (PLA2) activity was investigated in various tissues of tobacco (Nicotiana tabacum). PLA2 activity in the flower was 15 times higher than that in the leaf, stem, and root. PLA2 activity in the flo...

    Ritsuko Fujikawa, Yukichi Fujikawa, Noriaki Iijima, Muneharu Esaka in Lipids (2005)

  6. No Access

    Article

    Generation and properties of ascorbic acid-overproducing transgenic tobacco cells expressing sense RNA for l-galactono-1,4-lactone dehydrogenase

    l-Galactono-1,4-lactone dehydrogenase (GalLDH; EC 1.3.2.3) is the last enzyme in the putative l-ascorbic acid (AsA) biosynthetic pathway of plants. Here, we show for the first time that the overexpression of GalL...

    Takaaki Tokunaga, Katsunori Miyahara, Kazufumi Tabata, Muneharu Esaka in Planta (2005)

  7. No Access

    Article

    Purification, characterization, and molecular cloning of group I phospholipases A2 from the gills of the red sea bream, Pagrus major

    Phospholipase A2 (PLA2) activity was investigated in various tissues of male and female red sea bream. In both male and female fishes, the specific activity of PLA2 in the gills was 70 times higher than that in o...

    Noriaki Iijima, Satoshi Uchiyama, Yukichi Fujikawa, Muneharu Esaka in Lipids (2000)

  8. No Access

    Article

    Expansion of transgenic tobacco protoplasts expressing pumpkin ascorbate oxidase is more rapid than that of wild-type protoplasts

     When pumpkin (Cucurbita spp., cv. Ebisu Nankin) ascorbate oxidase cDNA was introduced into cultured cells of tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow No. 2) by Agrobacterium-mediated transformation, ...

    Naohiro Kato, Muneharu Esaka in Planta (2000)

  9. No Access

    Article

    cDNA cloning and differential gene expression of three catalases in pumpkin

    Three cDNA clones (cat1, cat2, cat3) for catalase (EC 1.11.1.6) were isolated from a cDNA library of pumpkin (Cucurbita sp.) cotyledons. In northern blotting using the cDNA-specific probe, the cat1 mRNA levels...

    Muneharu Esaka, Naoko Yamada, Masao Kitabayashi, Yuji Setoguchi… in Plant Molecular Biology (1997)

  10. No Access

    Article

    cDNA cloning and gene expression of ascorbate oxidase in tobacco

    A cDNA clone for ascorbate oxidase (AAO) has been isolated from a cDNA library of tobacco (Nicotiana tabacum) cells. The identity of the amino acid sequence deduced from tobacco AAO cDNA to that from pumpkin AAO ...

    Naohiro Kato, Muneharu Esaka in Plant Molecular Biology (1996)

  11. No Access

    Chapter and Conference Paper

    Over-All Drying-Rate Equations of Foodstuffs

    Many studies on the drying-rate equations have been performed. They all divide the drying periods into a constant- and a falling-rate period. However, for several foodstuffs the drying phenomena are very compl...

    Kiyoshi Kubota, Kanichi Suzuki, Muneharu Esaka, Hideki Araki, Mitsuo Nagai in Drying ’85 (1985)

  12. No Access

    Chapter and Conference Paper

    Characteristics of Vibro-Fluidized Bed Freeze Drying

    The freeze drying of granular or chopped materials was carried out in a laboratory size vibro-fluidized bed. The heater, made of stainless steel pipe, was set in the bed for minimizing heat losses. General cha...

    Kanichi Suzuki, Masaaki Ikeda, Muneharu Esaka, Kiyoshi Kubota in Drying ’85 (1985)