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Article
Homologues of a vacuolar processing enzyme that are expressed in different organs in Arabidopsis thaliana
Vacuolar processing enzymes (VPEs) are responsible for the maturation of seed proteins. These processing enzymes belong to a novel group of cysteine proteinases with molecular masses of 37 to 39 kDa. We isolat...
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Article
Characterization of two integral membrane proteins located in the protein bodies of pumpkin seeds
Two integral membrane proteins, MP28 and MP23, were found in protein bodies isolated from pumpkin (Cucurbita sp.) seeds. Molecular characterization revealed that both MP28 and MP23 belong to the seed TIP (tonopla...
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Chapter
Molecular Characterization of Aconitase in Etiolated Pumpkin Cotyledons
Aconitase (EC 4.2.1.3) is an enzyme that catalyzes the reaction of reversible conversion between citrate and isocitrate. In cotyledons of oil-storage plants such as pumpkin, aconitase was thought to exist not ...
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Article
Molecular characterization of a glyoxysomal citrate synthase that is synthesized as a precursor of higher molecular mass in pumpkin
A cDNA clone for glyoxysomal citrate synthase (gCS) was isolated from a λgt11 cDNA library prepared from etiolated pumpkin cotyledons. The cDNA of 1989 bp consisted of a 1548 bp open reading frame that encoded...
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Article
The presequence of the precursor to the nucleus-encoded 30 kDa protein of photosystem II in Euglena gracilis Z includes two hydrophobic domains
A cDNA clone for the extrinsic 30 kDa protein (OEC30) of photosystem II in Euglena gracilis Z was isolated and characterized. The open reading frame of the cDNA encoded a polypeptide of 338 amino acids, which con...
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Article
Molecular structure and subcellular localization of spinach leaf glycolate oxidase
Glycolate oxidase (E.C. 1.1.3.1) was purified from spinach leaves (Spinacia oleracea). The molecular weight of the native protein was determined by sucrose density gradient centrifugation to be 290,000 daltons (1...
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Article
Molecular structure and subcellular localization of spinach leaf glycolate oxidase
Glycolate oxidase (E.C. 1.1.3.1) was purified from spinach leaves (Spinacia oleracea). The molecular weight of the native protein was determined by sucrose density gradient centrifugation to be 290,000 daltons (1...
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Article
Hydrolysis of protein in vacuoles isolated from higher plant tissue
THE vacuoles of higher plant cells are known to be sites of deposition of salts and metabolites. A role as the lytic compartment has also been proposed1, and Matile has argued that the vacuole participates in the...
