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Article
Open AccessSelective gene expression profiling contributes to a better understanding of the molecular pathways underlying the histological changes observed after RHMVL
In previous studies, five vasoactive drugs were investigated for their effect on the recovery process after extended liver resection without observing relevant improvements. We hypothesized that an analysis of...
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Article
Open AccessThe formation of estrogen-like tamoxifen metabolites and their influence on enzyme activity and gene expression of ADME genes
Tamoxifen, a standard therapy for breast cancer, is metabolized to compounds with anti-estrogenic as well as estrogen-like action at the estrogen receptor. Little is known about the formation of estrogen-like ...
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Article
Open AccessEthanol sensitizes hepatocytes for TGF-β-triggered apoptosis
Alcohol abuse is a global health problem causing a substantial fraction of chronic liver diseases. Abundant TGF-β—a potent pro-fibrogenic cytokine—leads to disease progression. Our aim was to elucidate the cro...
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Article
Open AccessPathobiochemical signatures of cholestatic liver disease in bile duct ligated mice
Disrupted bile secretion leads to liver damage characterized by inflammation, fibrosis, eventually cirrhosis, and hepatocellular cancer. As obstructive cholestasis often progresses insidiously, markers for the...
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Article
Open AccessVirtual pathway explorer (viPEr) and pathway enrichment analysis tool (PEANuT): creating and analyzing focus networks to identify cross-talk between molecules and pathways
Interpreting large-scale studies from microarrays or next-generation sequencing for further experimental testing remains one of the major challenges in quantitative biology. Combining expression with physical ...
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Article
Open AccessThe truncated splice variant of peroxisome proliferator-activated receptor alpha, PPARα-tr, autonomously regulates proliferative and pro-inflammatory genes
The peroxisome proliferator-activated receptor alpha (PPARα) controls lipid/energy homeostasis and inflammatory responses. The truncated splice variant PPARα-tr was suggested to exert a dominant negative funct...
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Article
Open AccessTargeted epigenome editing of an endogenous locus with chromatin modifiers is not stably maintained
DNA methylation and histone 3 lysine 9 (H3K9) methylation are considered as epigenetic marks that can be inherited through cell divisions. To explore the functional consequences and stability of these modifica...
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Article
Open AccessA novel reverse transduction adenoviral array for the functional analysis of shRNA libraries
The identification of novel drug targets by assessing gene functions is most conveniently achieved by high-throughput loss-of-function RNA interference screening. There is a growing need to employ primary cell...
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Article
Targeting leukemic fusion proteins with small interfering RNAs: recent advances and therapeutic potentials
RNA interference has become an indispensable research tool to study gene functions in a wide variety of organisms. Because of their high efficacy and specificity, RNA interference-based approaches may also tra...
