59 Result(s)
within
Isao Karube
Biotechnology
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Reference Work Entry In depth
Organic Matter BOD Biosensor Monitoring
From a proposal of a microbial Biochemical Oxygen Demand (BOD) biosensor by Karube et al. in 1977, components (recognition elements, transducer, etc.) have so far been developed from various perspectives such ...
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Living Reference Work Entry In depth
Organic Matter BOD Biosensor Monitoring
From a proposal of a microbial Biochemical Oxygen Demand (BOD) biosensor by Karube et al. in 1977, components (recognition elements, transducer, etc.) have so far been developed from various perspectives such ...
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Chapter
Yeast Based Sensors
Since the first microbial cell sensor was studied by Karube et al. in 1977, many types of yeast based sensors have been developed as analytical tools. Yeasts are known as facultative anaerobes. Facultative ana...
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Chapter
Applications of Microbial Cell Sensors
Since the first microbial cell sensor was studied by Karube et al. in 1977, many types of microbial cell sensors have been developed as analytical tools. The microbial cell sensor utilizes microbes as a sensin...
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Chapter
Development of Microbial Sensors and Their Application
Many types of microbial sensors have been developed as analytical tools since the first microbial sensor was studied by Karube et al. in 1977. The microbial sensor consists of a transducer and microbe ...
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Article
Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis
A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...
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Article
Isolation of a bacterium from mangrove soil for degradation of sea sludge
Sea sludge, which is sediment of fish excrement and sewage on the sea bottom, continues to be a serious environmental problem. It has the potential to cause eutrophication and red tide, resulting in the death ...
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Article
Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation
Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compoun...
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Article
Purification of single stranded DNA from asymmetric PCR product using the SMART system
A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric PCR produ...
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Article
Enzymatic screening of microalgae as a potential source of natural antioxidants
Extracts of over 100 species of microalgae both from nature and from laboratory cultures were screened for antioxidant activity. As an assay for antioxidant function, we examined the inhibition of the activity...
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Article
Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts
Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the re...
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Article
Determination of cyanide using a microbial sensor
A microbial cyanide sensor was prepared, consisting of immobilizedSaccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of ...
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Article
A novel synthesis method for cyclodextrins from maltose in water-organic solvent systems
A novel enzymatic synthesis method of cyclodextrin (CD) from low-mol-wt maltose using cyclomaltodextrin glucanotransferase (CGTase) fromBacillus macerans has been developed in various water-organic solvent system...
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Article
Enzymatic hydrolysis of starch in water-immiscible organic solvent, two-phase systems
Enzyme-catalyzed hydrolyzations of starch by α-amylase have been studied in various two-phase systems, consisting of water and a water-immiscible organic solvent. The hydrolytic conversion of soluble starch to...
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Article
Continuous two step bioconversion of 4-oxoiso-phorone (OIP) to 4-hydroxy-2,2,6-trimethylcyclo-hexanone (4-HTMCH) by thermophilic bacteria
The continuous two step conversion of 4-oxoisophorone(OIP) to 4-hydroxy-2,2,6-trimethylcyclohexanone(4-HTMCH) via dihydrooxoisophorone(DOIP) was carried out using two types of thermophilic growing cells, Thermomo...
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Article
Influence of cationic antibiotics on phase behavior of rough-form lipopolysaccharide
The rough-form lipopolysaccharide (LPS) interacted with cationic antibiotic polymyxin B and gramicidin S in solution, and showed altered thermotropic phase behavior and viscoelasticity. The phase behavior was ...
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Article
Research and development of biosensors in Japan
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Chapter and Conference Paper
Development of Micro—Biosensors for Brain Research
Neurotransmitters play important roles in the brain since they are the key link in communication between neurons. Acetylcholine is the transmitter of motor neurons in the spinal cord as well as in all the nerv...
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Article
A biocompatible needle-type glucose sensor based on platinum-electroplated carbon electrode
A biocompatible needle-type glucose sensor with a 3-electrode configuration was constructed. A platinum-electroplated carbon stick was used as the working electrode, Ag/AgCl as the reference electrode, and a d...
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Article
Structural characterization of lipid A component of Erwinia carotovora lipopolysaccharide
The chemical structure of the lipid A component of lipopolysaccharide excreted into the liquid medium by the plant pathogenic enterobacterium Erwinia carotovora FERM P-7576 was characterized. It consists of a β-1...
