33 Result(s)

Article

Hydrogels Based on Recombinant Spidroin Stimulate Proliferation and Migration of Human Corneal Cells

The effect of recombinant spidroin (RS) hydrogel (HG) on anterior epithelial cells and keratocytes of the human cornea was studied in vitro. Corneal injuries are highly prevalent in develo** countries accord...

O. I. Agapova, D. S. Ostrovsky, M. Kh. Khubetsova… in Doklady Biological Sciences (2023)

Article

Identification of Ultrastructural Details of the Astrocyte Process System in Nervous Tissue of the Brain Using Correlative Scanning Probe and Transmission Electron Microscopy

Nanoscale morphological features of branched processes of glial cells may be of decisive importance for neuron–astrocyte interactions in health and disease. The paper presents the results of a correlation anal...

O. I. Agapova, A. E. Efimov, E. A. Obraztsova… in Doklady Biological Sciences (2023)

Article

Correlative Fluorescent Scanning Probe Nanotomography Used to Study the Intracellular Distribution of Doxorubicin in MCF-7 Human Breast Adenocarcinoma Cells

Develo** technologies for efficient targeted drug delivery for oncotherapy requires new methods to analyze the features of micro- and nanoscale distributions of antitumor drugs in cells and tissues. A new ap...

O. I. Agapova, A. E. Efimov, K. E. Mochalov, D. O. Solovyeva… in Doklady Biological Sciences (2023)

Article

Investigation of the Distribution of Magnetic Nanoparticles in Tumor Tissues by the Method of Scanning Magnetic Force Nanotomography

The development of effective biomedical technologies using magnetic nanoparticles (MNPs) for the tasks of oncotherapy and nanodiagnostics requires the development and implementation of new methods for the anal...

O. I. Agapova, A. E. Efimov, K. E. Mochalov… in Doklady Biochemistry and Biophysics (2022)

Article

Scanning Optical Probe Nanotomography for Investigation of the Structure of Biomaterials and Cells

Creation of new effective bio-artificial structures for tissue engineering and regenerative medicine requires development and implementation of new technological approaches for analysis of micro- and nanostruc...

O. I. Agapova, A. E. Efimov, L. A. Safonova… in Doklady Biochemistry and Biophysics (2021)

Article

Surface Modification of Alginate Microcarriers for Improvement of Their Biological Properties

The obtaining of microcarriers for the cell culture and delivery is an urgent task of tissue engineering and regenerative medicine. The novel method of surface modification of alginate microcarriers in the for...

L. A. Safonova, M. M. Bobrova, A. E. Efimov, O. I. Agapova… in Doklady Biological Sciences (2021)

Article

Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers

We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the...

A. V. Goncharenko, M. S. Kotlyarova… in Doklady Biochemistry and Biophysics (2017)

Article

Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration

Using the tissue-engineered constructs based on scaffolds that imitate the extracellular matrix of living tissues unveils new opportunities in the treatment of various pathologies and injuries associated with ...

M. S. Kotliarova, A. Yu. Arkhipova… in Moscow University Biological Sciences Bull… (2017)

Article

Regeneration of jejunal wall defect using an implant based on silk fibroin fibers

Regenerative properties of fibroin implant vitalized with allogeneic bone marrow cells were assessed. The study was performed using the experimental model of rat jejunum wall damage. Three weeks after surgery,...

M. M. Moisenovich, D. A. Kulikov, A. V. Goncharenko… in Doklady Biochemistry and Biophysics (2017)

Article

Effects of fibroin microcarriers on inflammation and regeneration of deep skin wounds in mice

The process of tissue regeneration following damage takes place with direct participation of the immune system. The use of biomaterials as scaffolds to facilitate healing of skin wounds is a new and interestin...

A. Y. Arkhipova, M. A. Nosenko, N. V. Malyuchenko, R. V. Zvartsev… in Biochemistry (Moscow) (2016)

Article

Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds

3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associa...

A. V. Goncharenko, N. V. Malyuchenko… in Doklady Biochemistry and Biophysics (2016)

Article

Recombinant 1F9 spidroin microgels for murine full-thickness wound repairing

The study of the stimulating effect of the microgels (MGs) based on recombinant 1F9 spidroin on the regeneration of the deep skin wound in mice was carried out. The use of spidroin MGs was shown to increase si...

M. M. Moisenovich, N. V. Malyuchenko… in Doklady Biochemistry and Biophysics (2016)

Article

Novel 3D-microcarriers from recombinant spidroin for regenerative medicine

Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with ...

M. M. Moisenovich, N. V. Malyuchenko… in Doklady Biochemistry and Biophysics (2015)

Article

Characterization of biodegradable cell micro and macro carriers based on recombinant spidroin

Gels, microgels, and matrices were prepared based on a previously developed recombinant spidroin, 1F9, produced by Saccharomyces cerevisiae yeast strain; their physical, chemical, and biological properties were i...

V. G. Bogush, L. I. Davydova, M. M. Moisenovich… in Applied Biochemistry and Microbiology (2014)

Article

Effect of ricin on photodynamic damage to the plasma membrane

M. M. Moisenovich, I. I. Agapov, A. A. Ramonova… in Doklady Biochemistry and Biophysics (2013)

Article

Boronated derivatives of chlorin e ₆ and fluoride-containing porphyrins as penetrating anions: a study using bilayer lipid membranes

Boronated derivatives of porphyrins are studied extensively as promising compounds for boron-neutron capture therapy and photodynamic therapy. Understanding of the mechanism of their permeation across cell mem...

T. I. Rokitskaya, A. V. Zaitsev, V. A. Ol’shevskaya, V. N. Kalinin… in Biochemistry (Moscow) (2012)

Article

Recombinant analogue of spidroin 2 for biomedical materials

V. G. Bogush, K. V. Sidoruk, L. I. Davydova… in Doklady Biochemistry and Biophysics (2011)

Article

Biocomposite scaffolds containing regenerated silk fibroin and nanohydroxyapatite for bone tissue regeneration

I. I. Agapov, M. M. Moisenovich, T. V. Druzhinina… in Doklady Biochemistry and Biophysics (2011)

Article

Biocompatible materials from regenerated silk for tissue engineering and medicinal therapy

The present review is devoted to the application of biomaterials from regenerated silk for designing tissue-engineered constructs—the basis for hybrid organs and tissues. Fibroin, the main structural protein o...

A. S. Kon’kov, O. L. Pustovalova, I. I. Agapov in Applied Biochemistry and Microbiology (2010)