Abstract
We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the large surface area, and the contribution of microcarrier mineralization to the stimulation of the osteogenic differentiation of mesenchymal stem cells was revealed.
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Rohban, R. and Pieber, T.R., Mesenchymal stem and progenitor cells in regeneration: tissue specificity and regenerative potential, Stem Cells Int., 2017, vol. 2017, p. 5173732.
Squillaro, T., Peluso, G., and Galderisi, U., Clinical trials with mesenchymal stem cells: an update, Cell Transplant., 2016, vol. 25, no. 5, pp. 829–848.
Phinney, D.G. and Prockop, D.J., Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair-current views, Stem Cells, 2007, vol. 25, no. 11, pp. 2896–2902.
Caplan, A.I. and Correa, D., The MSC: an injury drugstore, Cell Stem Cell, 2011, vol. 9, no. 1, pp. 11–15.
Andreeva, N.V., Bonartsev, A.P., Zharkova, I.I., Makhina, T.K., Myshkina, V.L., Kharitonova, E.P., Voinova, V.V., Bonartseva, G.A., Shaitan, K.V., and Belyavskii, A.V., Culturing mouse mesenchymal stem cells on poly-3-oxybutyrate carriers, Klet. Tekhnol. Biol. Med., 2015, no. 2, pp. 114–119.
Goncharenko, A.V., Malyuchenko, N.V., Moisenovich, A.M., Kotlyarova, M.S., Arkhipova, A.Yu., Kon’kov, A.S., Agapov, I.I., Molochkov, A.V., Moisenovich, M.M., and Kirpichnikov, M.P., Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast- like cells on mineralized fibroin scaffolds, Dokl. Biochem. Biophys., 2016, vol. 470, pp. 368–370.
Moisenovich, M.M., Kulikov, D.A., Arkhipova, A.Yu., Malyuchenko, N.V., Kotlyarova, M.S., Goncharenko, A.V., Kulikov, A.V., Mashkov, A.E., Agapov, I.I., Paleev, F.P., Svistunov, A.A., and Kirpichnikov, M.P., Fundamentals of the use of fibroin silk-based bioresorbable microcarriers in therapeutic practice: A case study of skin regeneration, Terapevt. Arkhiv, 2015, vol. 87, no. 12, pp. 66–72.
Huang, S., Xu, L., Sun, Y., Wu, T., Wang, K., and Li, G., An improved protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow, J. Orthop. Transl., 2015, vol. 3, no. 1, pp. 26–33.
Jung, G.Y., Park, Y.J., and Han, J.S., Effects of ha released calcium ion on osteoblast differentiation, J. Mater. Sci. Mater. Med., 2010, vol. 21, no. 5, pp. 1649–1654.
Viti, F., Landini, M., Mezzelani, A., Petecchia, L., Milanesi, L., and Scaglione, S., Osteogenic differentiation of MSC through calcium signaling activation: transcriptomics and functional analysis, PLoS One, 2016, vol. 11, no. 2, p. e0148173.
Luegmayr, E., Varga, F., Frank, T., Roschger, P., and Klaushofer, K., Effects of triiodothyronine on morphology, growth behavior, and the actin cytoskeleton in mouse osteoblastic cells (MC3T3-E1), Bone, 1996, vol. 18, no. 6, pp. 591–599.
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Original Russian Text © A.V. Goncharenko, M.S. Kotlyarova, A.M. Moisenovich, A.Y. Arkhipova, D.A. Kulikov, A.S. Konkov, A.V. Kulikov, A.E. Mashkov, I.I. Agapov, M.M. Moisenovich, M.P. Kirpichnikov, 2017, published in Doklady Akademii Nauk, 2017, Vol. 477, No. 1, pp. 97–100.
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Goncharenko, A.V., Kotlyarova, M.S., Moisenovich, A.M. et al. Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers. Dokl Biochem Biophys 477, 345–348 (2017). https://doi.org/10.1134/S1607672917060011
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DOI: https://doi.org/10.1134/S1607672917060011