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Scanning Optical Probe Nanotomography for Investigation of the Structure of Biomaterials and Cells

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Creation of new effective bio-artificial structures for tissue engineering and regenerative medicine requires development and implementation of new technological approaches for analysis of micro- and nanostructural features of constructs based on biomaterials and their interaction with cells. A new method of three-dimensional multiparametric analysis of nanostructure, scanning optical probe nanotomography, is presented in this paper, applied to the analysis of cells and biomaterials. Correlative reconstruction of fluorescent marker distributions and nanostructure features allows quantitative evaluation of a number of parameters of three-dimensional nanomorphology of fibroblasts and human hepatocarcinoma cells Hep-G2, adhered to biodegradable scaffolds based on silk fibroin. The developed technology with use of scanning optical probe nanotomography is applicable to investigation of three-dimensional micro- and nanostructure features of biomaterials and cells of different types.

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Funding

This work was partially supported by the program of the President of the Russian Federation for state support of leading scientific schools of the Russian Federation (project no. NSh-2598.2020.7).

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Authors and Affiliations

  1. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Moscow, Russia

    O. I. Agapova, A. E. Efimov, L. A. Safonova, M. M. Bobrova, I. I. Agapov &  S. V. Gautier

  2. Sechenov University, Moscow, Russia

    S. V. Gautier

Authors
  1. O. I. Agapova
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  2. A. E. Efimov
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  3. L. A. Safonova
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  4. M. M. Bobrova
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  5. I. I. Agapov
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  6. S. V. Gautier
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Corresponding author

Correspondence to I. I. Agapov.

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The authors declare that they have no conflict of interests. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Agapova, O.I., Efimov, A.E., Safonova, L.A. et al. Scanning Optical Probe Nanotomography for Investigation of the Structure of Biomaterials and Cells. Dokl Biochem Biophys 500, 331–334 (2021). https://doi.org/10.1134/S160767292105001X

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  • DOI: https://doi.org/10.1134/S160767292105001X

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