-
Article
CRISPR Interference in Regulation of Bacterial Gene Expression
The review is devoted to the use of the CRISPR/Cas system for obtaining knockdowns of target bacterial genes by CRISPR-mediated interference (CRISPRi). CRISPRi is based on the preservation of the ability of th...
-
Article
Open AccessGenetic Engineering in Mycobacteria
Genetic tools for targeted modification of the mycobacterial genome contribute to the understanding of the physiology and virulence mechanisms of mycobacteria. Human and animal pathogens, such as the Mycobacteriu...
-
Article
CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis
This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA sp...
-
Article
Vaccines Against Tuberculosis: Problems and Prospects (Review)
Despite the efforts of the global medical and scientific community, tuberculosis remains the leading cause of death from infectious diseases. The expectation of success associated with the development of new a...
-
Article
Biotechnological Potential of the Bacillus subtilis 20 Strain
Bacillus subtilis bacteria play an important role in veterinary medicine, medicine, and biotechnology, and the permanently growing demand for biotechnological products fuels the improvement of the properties of b...
-
Article
Toxin-Antitoxin Systems and Bacterial Persistence (Review)
Persistence is a phenomenon in which bacteria avoid the lethal effects of antibiotics due to their transition to a physiological state, which enables tolerance to antibacterial drugs. Among the factors that co...
-
Article
cAMP-Dependent Transcription Factor in Mycobacterium tuberculosis Coded by the Rv3676 Gene as a Possible Target for the Development of Antituberculosis Drugs
Adenylate cyclase Rv2212 plays a key role in the growth of Mycobacterium tuberculosis (Mtb) and its reactivation from the dormant state. This enzyme could potentially be a good target for the development of new a...
-
Article
Osteogenic differentiation of mouse bone marrow stromal cells on fibroin microcarriers
We investigated the proliferation and osteogenic differentiation of mesenchymal stem cells cultured on fibroin microcarriers. Effective cell proliferation on the surface of the microcarriers, determined by the...
-
Article
Bioresorbable Scaffolds Based on Fibroin for Bone Tissue Regeneration
Using the tissue-engineered constructs based on scaffolds that imitate the extracellular matrix of living tissues unveils new opportunities in the treatment of various pathologies and injuries associated with ...
-
Article
Regeneration of jejunal wall defect using an implant based on silk fibroin fibers
Regenerative properties of fibroin implant vitalized with allogeneic bone marrow cells were assessed. The study was performed using the experimental model of rat jejunum wall damage. Three weeks after surgery,...
-
Article
VapC toxin inhibition as a method for prevention of the formation of resting forms of mycobacteria
It has been shown that inactivation of the VapC toxin from the VapВС toxin-antitoxin system prevents mycobacterial cells from transitioning to an ovoid state that meets the criteria of dormancy. The results in...
-
Article
Induction of osteogenic differentiation of osteoblast-like cells MG-63 during cultivation on fibroin microcarriers
We have developed microcarriers made from silk fibroin. Microcarriers can be used as a substrate for cell cultivation and cell delivery during cell-based therapy and for the construction of bioengineered tissu...
-
Article
Changes in morphology of actin filaments and expression of alkaline phosphatase at 3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds
3D cultivation of MG-63 osteoblast-like cells on mineralized fibroin scaffolds leads to an increase in the expression of alkaline phosphatase, an early marker of bone formation. Increased expression is associa...
-
Article
Recombinant 1F9 spidroin microgels for murine full-thickness wound repairing
The study of the stimulating effect of the microgels (MGs) based on recombinant 1F9 spidroin on the regeneration of the deep skin wound in mice was carried out. The use of spidroin MGs was shown to increase si...
-
Article
Novel 3D-microcarriers from recombinant spidroin for regenerative medicine
Microcarriers generated from recombinant spidroin 1F9 are suitable for use as an injection material. The microcarriers were a heterogeneous mixture of microgel particles ranging from 50 to 300 µm in size with ...
-
Article
Bacterial toxin-antitoxin systems and perspectives for their application in medicine
The structure of various toxin-antitoxin (TA) families and the principles of their action are reviewed. TA loci are widely distributed in the genomes of eubacteria and archaea. Most TA systems are two-componen...
-
Article
Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria
Artificial generation of oxygen superoxide radicals in actively growing cultures of Mycobacterium tuberculosis, Myc. smegmatis, and Corynebacterium ammoniagenes is followed by accumulation in the bacterial cells ...
-
Article
Histone-like proteins of bacteria (review)
Four major families of bacterial histone-like proteins (HU, IHF, H-NS, FIS), united on the basis of structural similarity and performing specific structural and regulatory functions in the cell, are discussed....
-
Article
The model of resting forms of micobacteria for testing of chemodrugs for latent forms of tuberculosis
The new model of obtaining of ovoid resting forms of Mycobacterium smegmatis, which are morphologically different from vegetative (rod-like) cells, was developed. Ovoid forms were characterized by a drastically d...
-
Article
Nitroxyl compounds in bacteria: Search for functions
A method was developed for incorporation of two deuterium atoms into the reactive group of a new type of nitroxyl compounds from Micrococcus luteus (a lysodektose which can be transformed into a long-living free ...
