9 Result(s)
within
Flavie Lavoie-Cardinal
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Article
Open AccessResolution enhancement with a task-assisted GAN to guide optical nanoscopy image analysis and acquisition
Super-resolution fluorescence microscopy methods enable the characterization of nanostructures in living and fixed biological tissues. However, they require the adjustment of multiple imaging parameters while ...
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Article
Microscopy analysis neural network to solve detection, enumeration and segmentation from image-level annotations
The development of deep learning approaches to detect, segment or classify structures of interest has transformed the field of quantitative microscopy. High-throughput quantitative image analysis presents a ch...
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Article
Open AccessA postzygotic de novo NCDN mutation identified in a sporadic FTLD patient results in neurochondrin haploinsufficiency and altered FUS granule dynamics
Frontotemporal dementia (FTD) is a heterogeneous clinical disorder characterized by progressive abnormalities in behavior, executive functions, personality, language and/or motricity. A neuropathological subty...
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Protocol
Automated Microscopy Image Segmentation and Analysis with Machine Learning
The development of automated quantitative image analysis pipelines requires thoughtful considerations to extract meaningful information. Commonly, extraction rules for quantitative parameters are defined and a...
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Article
Open AccessNeuronal activity remodels the F-actin based submembrane lattice in dendrites but not axons of hippocampal neurons
The nanoscale organization of the F-actin cytoskeleton in neurons comprises membrane-associated periodical rings, bundles, and longitudinal fibers. The F-actin rings have been observed predominantly in axons b...
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Article
Open AccessA machine learning approach for online automated optimization of super-resolution optical microscopy
Traditional approaches for finding well-performing parameterizations of complex imaging systems, such as super-resolution microscopes rely on an extensive exploration phase over the illumination and acquisitio...
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Protocol
Nanoparticle-Assisted Localized Optical Stimulation of Cultured Neurons
Nanoparticle-assisted localized optical stimulation (NALOS) of cultured neurons is an all-optical method that allows subcellular light stimulation to investigate localized signaling in neurons. The stimulation...
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Article
Open AccessGold nanoparticle-assisted all optical localized stimulation and monitoring of Ca2+ signaling in neurons
Light-assisted manipulation of cells to control membrane activity or intracellular signaling has become a major avenue in life sciences. However, the ability to perform subcellular light stimulation to investi...
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Article
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence cap...
