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Article
Open AccessMutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease
The polyglutamine expansion of huntingtin (mHTT) causes Huntington disease (HD) and neurodegeneration, but the mechanisms remain unclear. Here, we found that mHtt promotes ribosome stalling and suppresses prot...
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Article
Open AccessQuantitative optical nanophysiology of Ca2+ signaling at inner hair cell active zones
Ca2+ influx triggers the release of synaptic vesicles at the presynaptic active zone (AZ). A quantitative characterization of presynaptic Ca2+ signaling is critical for understanding synaptic transmission. Howeve...
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Article
Open AccessSRpHi ratiometric pH biosensors for super-resolution microscopy
Fluorescence-based biosensors have become essential tools for modern biology, allowing real-time monitoring of biological processes within living cells. Intracellular fluorescent pH probes comprise one of the ...
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Article
Coordinate-targeted fluorescence nanoscopy with multiple off states
Far-field super-resolution fluorescence microscopy discerns fluorophores residing closer than the diffraction barrier by briefly transferring them in different (typically ON and OFF) states before detection. I...
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Article
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence cap...
