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  1. Article

    Open Access

    Achromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy

    Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a f...

    Andriy Chmyrov, Marcel Leutenegger, Tim Grotjohann, Andreas Schönle… in Scientific Reports (2017)

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  2. No Access

    Article

    Nanoscopy with more than 100,000 'doughnuts'

    Two incoherently superimposed orthogonal standing waves are used to create a pattern of 116,000 'doughnuts' for fast, highly parallelized coordinate-targeted super-resolution microscopy of living cells, with a...

    Andriy Chmyrov, Jan Keller, Tim Grotjohann, Michael Ratz, Elisa d'Este… in Nature Methods (2013)

  3. No Access

    Article

    Diffraction-unlimited all-optical imaging and writing with a photochromic GFP

    Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence cap...

    Tim Grotjohann, Ilaria Testa, Marcel Leutenegger, Hannes Bock, Nicolai T. Urban… in Nature (2011)

  4. No Access

    Article

    A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching

    Brakemann et al. present a reversibly photoswitchable fluorescent protein, called Dreiklang, that can be turned on and off at wavelengths distinct from those used for imaging. They show that the protein is advant...

    Tanja Brakemann, Andre C Stiel, Gert Weber, Martin Andresen… in Nature Biotechnology (2011)