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Article
Open AccessMINSTED nanoscopy enters the Ångström localization range
Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We bui...
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Article
Author Correction: MINSTED fluorescence localization and nanoscopy
A Correction to this paper has been published: https://doi.org/10.1038/s41566-021-00816-9.
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Article
Open AccessMINSTED fluorescence localization and nanoscopy
We introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale...
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Article
Open AccessAchromatic light patterning and improved image reconstruction for parallelized RESOLFT nanoscopy
Fluorescence microscopy is rapidly turning into nanoscopy. Among the various nanoscopy methods, the STED/RESOLFT super-resolution family has recently been expanded to image even large fields of view within a f...
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Article
Open AccessComplementarity of PALM and SOFI for super-resolution live-cell imaging of focal adhesions
Live-cell imaging of focal adhesions requires a sufficiently high temporal resolution, which remains a challenge for super-resolution microscopy. Here we address this important issue by combining photoactivate...
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Article
Diffraction-unlimited imaging: from pretty pictures to hard numbers
Diffraction-unlimited fluorescence imaging allows the visualization of intact, strongly heterogeneous systems at unprecedented levels of detail. Beyond the acquisition of detailed pictures, increasing efforts ...
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Article
Open AccessLive-cell multiplane three-dimensional super-resolution optical fluctuation imaging
Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinkin...
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Article
Open AccessMap** molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)
Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to local...
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Chapter and Conference Paper
Triplet Imaging of Oxygen Consumption During the Contraction of a Single Smooth Muscle Cell (A7r5)
Triplet imaging is a novel optical technique that allows investigating oxygen metabolism at the single cell and the sub-cellular level. The method combines high temporal and spatial resolutions which are requi...
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Article
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence cap...
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Article
A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching
Brakemann et al. present a reversibly photoswitchable fluorescent protein, called Dreiklang, that can be turned on and off at wavelengths distinct from those used for imaging. They show that the protein is advant...
