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Article
Open AccessMolecular recognition of a membrane-anchored HIV-1 pan-neutralizing epitope
Antibodies against the carboxy-terminal section of the membrane-proximal external region (C-MPER) of the HIV-1 envelope glycoprotein (Env) are considered as nearly pan-neutralizing. Development of vaccines cap...
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Article
Open AccessMultiscale interactome analysis coupled with off-target drug predictions reveals drug repurposing candidates for human coronavirus disease
The COVID-19 pandemic has highlighted the urgent need for the identification of new antiviral drug therapies for a variety of diseases. COVID-19 is caused by infection with the human coronavirus SARS-CoV-2, wh...
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Article
Open AccessMultivalency transforms SARS-CoV-2 antibodies into ultrapotent neutralizers
SARS-CoV-2, the virus responsible for COVID-19, has caused a global pandemic. Antibodies can be powerful biotherapeutics to fight viral infections. Here, we use the human apoferritin protomer as a modular subu...
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Article
Open AccessStructural characterization of the ICOS/ICOS-L immune complex reveals high molecular mimicry by therapeutic antibodies
The inducible co-stimulator (ICOS) is a member of the CD28/B7 superfamily, and delivers a positive co-stimulatory signal to activated T cells upon binding to its ligand (ICOS-L). Dysregulation of this pathway ...
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Article
Open AccessMolecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions
Antibodies against the Membrane-Proximal External Region (MPER) of the Env gp41 subunit neutralize HIV-1 with exceptional breadth and potency. Due to the lack of knowledge on the MPER native structure and acce...
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Article
Open AccessStructural basis for broad neutralization of HIV-1 through the molecular recognition of 10E8 helical epitope at the membrane interface
The mechanism by which the HIV-1 MPER epitope is recognized by the potent neutralizing antibody 10E8 at membrane interfaces remains poorly understood. To solve this problem, we have optimized a 10E8 peptide ep...
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Article
Open AccessOptimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels
In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplas...
