8 Result(s)
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Article
SViMULATE: a computer program facilitating interactive, multi-mode simulation of analytical ultracentrifugation data
The ability to simulate sedimentation velocity (SV) analytical ultracentrifugation (AUC) experiments has proved to be a valuable tool for research planning, hypothesis testing, and pedagogy. Several options fo...
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Article
Open AccessCorrection to: Reproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
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Article
Open AccessReproducibility and accuracy of microscale thermophoresis in the NanoTemper Monolith: a multi laboratory benchmark study
Microscale thermophoresis (MST), and the closely related Temperature Related Intensity Change (TRIC), are synonyms for a recently developed measurement technique in the field of biophysics to quantify biomolec...
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Article
Using modern approaches to sedimentation velocity to detect conformational changes in proteins
It has been known for decades that proteins undergo conformational changes in response to binding ligands. Such changes are usually accompanied by a loss of entropy by the protein, and thus conformational chan...
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Structure and ligand-binding mechanism of the human OX1 and OX2 orexin receptors
Human orexin receptors (hOX1R and hOX2R) are GPCRs involved in sleep regulation. Structures of hOX1R bound to a selective antagonist or to a dual antagonist, functional assays and computational analyses reveal th...
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Mechanism of actin filament nucleation by the bacterial effector VopL
VopL is a bacterial actin nucleation factor that induces actin stress fibers when injected by bacteria into eukaryotic host cells. Biochemical and structural analyses of VopL-mediated actin nucleation suggest ...
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Article
Structural basis of histone demethylation by LSD1 revealed by suicide inactivation
Histone methylation regulates diverse chromatin-templated processes, including transcription. The recent discovery of the first histone lysine–specific demethylase (LSD1) has changed the long-held view that hi...
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Side chain and backbone contributions of Phe508 to CFTR folding
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), an integral membrane protein, cause cystic fibrosis (CF). The most common CF-causing mutant, deletion of Phe508, fails to properly f...
