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    Article

    Side chain and backbone contributions of Phe508 to CFTR folding

    Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), an integral membrane protein, cause cystic fibrosis (CF). The most common CF-causing mutant, deletion of Phe508, fails to properly f...

    Patrick H Thibodeau, Chad A Brautigam in Nature Structural & Molecular Biology (2005)

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    Article

    Structural basis of histone demethylation by LSD1 revealed by suicide inactivation

    Histone methylation regulates diverse chromatin-templated processes, including transcription. The recent discovery of the first histone lysine–specific demethylase (LSD1) has changed the long-held view that hi...

    Maojun Yang, Jeffrey C Culhane in Nature Structural & Molecular Biology (2007)

  3. Article

    Open Access

    Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis

    Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing.

    Ahmed S Attia, Jennifer L Sedillo, Todd C Hoopman, Wei Liu, Lixia Liu in BMC Microbiology (2009)

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    Article

    Mechanism of actin filament nucleation by the bacterial effector VopL

    VopL is a bacterial actin nucleation factor that induces actin stress fibers when injected by bacteria into eukaryotic host cells. Biochemical and structural analyses of VopL-mediated actin nucleation suggest ...

    Bingke Yu, Hui-Chun Cheng, Chad A Brautigam in Nature Structural & Molecular Biology (2011)

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    Article

    Structure and ligand-binding mechanism of the human OX1 and OX2 orexin receptors

    Human orexin receptors (hOX1R and hOX2R) are GPCRs involved in sleep regulation. Structures of hOX1R bound to a selective antagonist or to a dual antagonist, functional assays and computational analyses reveal th...

    Jie Yin, Kerim Babaoglu, Chad A Brautigam in Nature Structural & Molecular Biology (2016)

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    Article

    Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions

    This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of resulting data ca...

    Chad A Brautigam, Huaying Zhao, Carolyn Vargas, Sandro Keller in Nature Protocols (2016)

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    Protocol

    Measuring the KD of Protein–Ligand Interactions Using Microscale Thermophoresis

    Microscale thermophoresis (MST) has become a widely used technique to determine the KD or EC50 of protein–ligand interactions. The method exploits the tendency of macromolecules to migrate along a thermal gradien...

    Shih-Chia Tso, Chad A. Brautigam in Protein-Ligand Interactions (2021)