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Protocol
Measuring the KD of Protein–Ligand Interactions Using Microscale Thermophoresis
Microscale thermophoresis (MST) has become a widely used technique to determine the KD or EC50 of protein–ligand interactions. The method exploits the tendency of macromolecules to migrate along a thermal gradien...
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Article
Integration and global analysis of isothermal titration calorimetry data for studying macromolecular interactions
This protocol from Brautigam et al. describes methods for baseline correction and global analysis of isothermal titration calorimetry data using NITPIC and SEDPHAT. Publication-quality graphs of resulting data ca...
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Article
Structure and ligand-binding mechanism of the human OX1 and OX2 orexin receptors
Human orexin receptors (hOX1R and hOX2R) are GPCRs involved in sleep regulation. Structures of hOX1R bound to a selective antagonist or to a dual antagonist, functional assays and computational analyses reveal th...
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Article
Mechanism of actin filament nucleation by the bacterial effector VopL
VopL is a bacterial actin nucleation factor that induces actin stress fibers when injected by bacteria into eukaryotic host cells. Biochemical and structural analyses of VopL-mediated actin nucleation suggest ...
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Article
Open AccessIdentification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis
Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing.
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Article
Structural basis of histone demethylation by LSD1 revealed by suicide inactivation
Histone methylation regulates diverse chromatin-templated processes, including transcription. The recent discovery of the first histone lysine–specific demethylase (LSD1) has changed the long-held view that hi...
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Article
Side chain and backbone contributions of Phe508 to CFTR folding
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR), an integral membrane protein, cause cystic fibrosis (CF). The most common CF-causing mutant, deletion of Phe508, fails to properly f...