7 Result(s)
within
Blanca López-Méndez
English
Science, Humanities and Social Sciences, multidisciplinary
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Article
Open AccessHalophilic enzyme activation induced by salts
Halophilic archea (halobacteriae) thrive in hypersaline environments, avoiding osmotic shock by increasing the ion concentration of their cytoplasm by up to 3–6 M. To remain folded and active, their constituti...
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Article
Open AccessConformational Selection and Induced Fit Mechanisms in the Binding of an Anticancer Drug to the c-Src Kinase
Understanding the conformational changes associated with the binding of small ligands to their biological targets is a fascinating and meaningful question in chemistry, biology and drug discovery. One of the m...
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Article
Open AccessBub1 positions Mad1 close to KNL1 MELT repeats to promote checkpoint signalling
Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Several aspects of Mad1/Mad2 kin...
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Article
Open AccessMolecular basis of Tousled-Like Kinase 2 activation
Tousled-like kinases (TLKs) are required for genome stability and normal development in numerous organisms and have been implicated in breast cancer and intellectual disability. In humans, the similar TLK1 and...
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Article
Open AccessUnderstanding the indirect DNA read-out specificity of I-CreI Meganuclease
The high DNA specificity of homing endonucleases makes them a powerful protein scaffold to engineer enzymes for genome manipulation. Understanding their molecular recognition of DNA is an important prerequisit...
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Article
Open AccessStructure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
Type III CRISPR-Cas multisubunit complexes cleave ssRNA and ssDNA. These activities promote the generation of cyclic oligoadenylate (cOA), which activates associated CRISPR-Cas RNases from the Csm/Csx families...
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Article
Open AccessStructure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon
CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opted CRISPR-Cas systems for RNA-guided transposition. Here we present the 2.4 Å cryo-EM structure of the Scytonema hofmannii (sh) TnsB tr...
