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Article
Open AccessStructure of the TnsB transposase-DNA complex of type V-K CRISPR-associated transposon
CRISPR-associated transposons (CASTs) are mobile genetic elements that co-opted CRISPR-Cas systems for RNA-guided transposition. Here we present the 2.4 Å cryo-EM structure of the Scytonema hofmannii (sh) TnsB tr...
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Article
Physical activity volume in patients with arrhythmogenic cardiomyopathy is associated with recurrence after ventricular tachycardia ablation
To assess the role of intense physical activity (PA) on recurrence after ventricular tachycardia (VT) ablation in arrhythmogenic cardiomyopathy (ACM).
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Article
Open AccessStructure of the mini-RNA-guided endonuclease CRISPR-Cas12j3
CRISPR-Cas12j is a recently identified family of miniaturized RNA-guided endonucleases from phages. These ribonucleoproteins provide a compact scaffold gathering all key activities of a genome editing tool. We...
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Article
Persistent pulmonary vein thrombosis
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Article
Open AccessStructure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas
Type III CRISPR-Cas multisubunit complexes cleave ssRNA and ssDNA. These activities promote the generation of cyclic oligoadenylate (cOA), which activates associated CRISPR-Cas RNases from the Csm/Csx families...
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Article
Class 2 CRISPR–Cas RNA-guided endonucleases: Swiss Army knives of genome editing
This review highlights recent mechanistic insights into the CRISPR class 2 type V enzymes Cpf1 and C2c1, which are crucial for improving these genome engineering tools and expanding the genomic editing space.
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Article
Erratum: Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
Nature 546, 559–563 (2017); doi:10.1038/nature22398 In Fig. 1b of this Letter, owing to an error during the production process, the 5′ and 3′ labels at the bottom of the structure diagram were inadvertently re...
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Article
Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage
The structure of Cpf1, a CRISPR–Cas/RNA-guided nuclease, is presented with a three-stranded RNA–DNA loop after cleavage, providing insight into its working mechanism.
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Article
Visualizing phosphodiester-bond hydrolysis by an endonuclease
Different catalytic steps of endonuclease I-DmoI are captured crystallographically to allow direct observation of the generation of a DNA double-strand break. A third metal ion enters the active site and has a...
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Repression of Transcription by Curved DNA and Nucleoid Protein H-NS
Nucleoid-associated protein H-NS has emerged as one of the most intriguing and versatile global regulators of enterobacterial gene expression acting primarily yet not exclusively at the transcriptional level w...
