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Article
R-loop hybridization of collagen DNA: Separation in Cs2SO4 gradients
High molecular weight poly(A)+ RNA (26–35 S) from chicken embryo trunks which is enriched for collagen mRNA was iodinated and hybridized to DNA under conditions of R-loop formation. The R-loops were separated in ...
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Article
Gluconeogenic-glycolytic capacities and metabolic zonation in liver of rats with streptozotocin, non-ketotic as compared to alloxan, ketotic diabetes
Activities (μmol x min−1 x g liver) and zonal distributions of key enzymes of carbohydrate metabolism were studied in livers of streptozotocin-diabetic rats and compared to the values in alloxan-diabetes.
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Article
Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents
The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing p...
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Chapter and Conference Paper
Die Bedeutung der Immunszintigraphie für die Diagnostik und Therapie gastrointestinaler Tumoren
Die Immunszintigraphie gastrointestinaler Tumoren besitzt bis heute ausschließlich Bedeutung für das kolorektale Karzinom, da für die übrigen Tumorgruppen keine entsprechenden Antikörper allgemein verfügbar si...
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Chapter and Conference Paper
Identification of G-Proteins Involved in the Inhibition of Ca2+ Currents in Neuronal and Endocrine Cells
Modulation of voltage-gated Ca2+ channels by hormones and neurotransmitters provides a common mechanism for regulation of cellular functions. During the last decade, the understanding of the underlying biochemica...
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Article
Different β-subunits determine G-protein interaction with transmembrane receptors
REGULATORY GTP-binding proteins (G proteins) are membrane-attached heterotrimers (α, β, γ) that mediate cellular responses to a wide variety of extracellular stimuli1,2. They undergo a cycle of guanine-nucleotide...
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Article
Enormous hemangiosarcoma of the heart
This report describes a 26-year-old patient with hemangiosarcoma of the heart and summarizes the clinicopathological features in previous reports of patients with cardiac angiosarcoma. The patient was admitted...
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Article
Interleukin-7, interleukin-12, and GM-CSF gene transfer in patients with metastatic melanoma
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Chapter
Immuntherapie
Immuntherapeutische Verfahren bei Patienten mit malignen Tumoren können in aktive und passive Ansätze unterteilt werden. Unter aktiver Immuntherapie versteht man den Versuch, das Immunsystem des Patienten unsp...
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Chapter
Functional Involvement of CD44, a Family of Cell Adhesion Molecules, in Immune Responses, Tumour Progression and Haematopoiesis
Cell-cell and cell-extracellular matrix (ECM) interactions play fundamental roles in many biological processes. Cells adhere to the ECM and to each other via specific cell surface receptors such as the selecti...
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Chapter
Inhibition of G Proteins by Antisense Drugs
In a multicellular organism, cells communicate with each other mostly through the cell surface, which is represented by the plasma membrane with its lipids and proteins. A subset of the membrane proteins, i.e....
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Article
Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3+CD56+ immunologic effector cells by receptor-mediated transfection with the interleukin-7 gene
Cytokine-induced killer (CIK) cells have been shown to eradicate established tumors in a SCID mouse–human lymphoma model. CIK cells depend on exogenous addition of cytokines such as interleukin-2 (IL-2), inter...
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Article
Vaccination with IL-12 gene-modified autologous melanoma cells: preclinical results and a first clinical phase I study
Cytokine gene transfer into tumor cells has been shown to mediate tumor regression and antimetastatic effects in several animal models via immunomodulation. Therefore, clinical protocols have been developed to...
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Article
Vaccination with IL-7 gene-modified autologous melanoma cells can enhance the anti-melanoma lytic activity in peripheral blood of patients with a good clinical performance status: a clinical phase I study
Recently, cytokine gene transfer into tumour cells has been shown to mediate tumour regression in animal models via immunomodulation. Consequently, a number of clinical protocols have been developed to treat c...
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Article
Regulation and possible function of axl expression in immature human mast cells
The receptor tyrosine kinase Axl which expresses extracellular domains reminiscent of cell adhesion molecules, is involved in homotypic binding as well as in intracellular signaling of myeloid progenitor cells. ...
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Article
Open AccessPhase I clinical study applying autologous immunological effector cells transfected with the interleukin-2 gene in patients with metastatic renal cancer, colorectal cancer and lymphoma
Natural killer-like T lymphocytes termed cytokine-induced killer (CIK) cells have been shown to eradicate established tumours in a severe combined immune deficient (SCID) mouse/human lymphoma model. Recently, ...
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Article
Ballistic transfer of minimalistic immunologically defined expression constructs for IL4 and CTLA4 into the corneal epithelium in mice after orthotopic corneal allograft transplantation
Background: Experiments were performed to determine whether corneal epithelium transfected with minimalistic immunologically defined expression constructs for the extracellular fragment of CTLA4 and for interleu...
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Article
Transfection of dendritic cells (DCs) with the CIITA gene: increase in immunostimulatory activity of DCs
Dendritic cells (DCs) are the major antigen-presenting cells. They are able to present tumor antigens to immunologic effector cells. MHC class II molecules on DC surfaces play an important role in priming effe...
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Article
Proliferation-dependent induction of apoptosis by the retinoid CD437 in p53-mutated keratinocytes
6-[3-(1-adamantyl)-4-hydroxyphenyl]-2-Naphthalene carboxylic acid (CD437) is a synthetic retinoid with strong apoptogenic properties in various neoplastic cell lines. CD437 was shown to induce apoptosis in ma...
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Article
The in vitro anti-inflammatory effects of recombinant anti-CD25 immunotoxin on lamina propria T cells of patients with inflammatory bowel disease are not sufficient to cure experimental colitis in mice
Background and aims: In chronic inflammatory bowel disease (IBD) such as Crohn's disease and ulcerative colitis an aberrant mucosal immune regulation is observed accompanied by upregulation of pr...
