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Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents

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Abstract

The inhibition of voltage-dependent Ca2+ channels in secretory cells by plasma membrane receptors is mediated by pertussis toxin-sensitive G proteins. Multiple forms of G proteins have been described, differing principally in their α subunits, but it has not been possible to establish which G-protein subtype mediates inhibition by a specific receptor. By intranuclear injection of antisense oligonucleotides into rat pituitary GH3 cells, the essential role of the Go-type G proteins in Ca2+-channel inhibition is established: the subtypes Go1 and Go2 mediate inhibition through the muscarinic and somatostatin receptors, respectively.

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References

  1. Birnbaumer, L. A. Rev. Pharmac. Toxicol. 30, 675–705 (1990).

    Article  CAS  Google Scholar 

  2. Simon, M. I., Strathmann, M. P. & Gautam, N. Science 252, 802–808 (1991).

    Article  ADS  CAS  Google Scholar 

  3. Graziano, M. P., Freissmuth, M. & Gilman, A. G. J. biol. Chem. 264, 409–418 (1989).

    CAS  PubMed  Google Scholar 

  4. Mattera, et al. J. biol. Chem. 264, 465–471 (1989).

    CAS  PubMed  Google Scholar 

  5. Asano, T., Semba, R., Kamiya, N., Ogasawara, N. & Kato, K. J. Neurochem. 50, 1164–1169 (1988).

    Article  CAS  Google Scholar 

  6. Hescheler, J., Rosenthal, W., Trautwein, W. & Schultz, G. Nature 325, 445–447 (1987).

    Article  ADS  CAS  Google Scholar 

  7. Schultz, G., Rosenthal, W., Trautwein, W. & Hescheler, J. A. Rev. Physiol. 52, 275–292 (1990).

    Article  CAS  Google Scholar 

  8. Offermanns, S. et al. Molec. Endocr. 5, 995–1002 (1991).

    Article  CAS  Google Scholar 

  9. Paulssen, E. J., Paulssen, R. H., Haugen, T. B., Gautvik, K. M. & Gordeladze, J. O. Molec. Cell. Endocr. 76, 45–53 (1991).

    Article  CAS  Google Scholar 

  10. Mumby, S. M., Kahn, R. A., Manning, D. R. & Gilman, A. G. Proc. natn. Acad. Sci. U.S.A. 83, 265–269 (1986).

    Article  ADS  CAS  Google Scholar 

  11. Offermanns, S., Schultz, G. & Rosenthal, W. J. biol. Chem. 266, 3365–3368 (1991).

    CAS  PubMed  Google Scholar 

  12. Rosenthal, W. et al. EMBO J. 7, 1627–1633 (1988).

    Article  CAS  Google Scholar 

  13. Cazenave, C. et al. Nucleic Acids Res. 17, 4255–4272 (1989).

    Article  CAS  Google Scholar 

  14. Zamecnik, P. C. & Stephenson, M. L. Proc. natn. Acad. Sci. U.S.A. 75, 280–284 (1978).

    Article  ADS  CAS  Google Scholar 

  15. Ewald, D. A., Sternweis, P. C. & Miller, R. J. Proc. natn. Acad. Sci. U.S.A. 85, 3633–3637 (1988).

    Article  ADS  CAS  Google Scholar 

  16. Harris-Warrick, R. M. et al. Neuron 1, 27–32 (1988).

    Article  CAS  Google Scholar 

  17. Toselli, M., Lang, J., Costa, T. & Lux, H. D. Pflügers Arch. ges. Physiol. 415, 255–261 (1989).

    Article  CAS  Google Scholar 

  18. McFadzean, I., Mullaney, I., Brown, D. A. & Milligan, G. Neuron 3, 177–182 (1989).

    Article  CAS  Google Scholar 

  19. Tsukamoto, T. et al. Proc. natn. Acad. Sci. U.S.A. 88, 2974–2978 (1991).

    Article  ADS  CAS  Google Scholar 

  20. Bertrand, P., Sanford, J., Rudolph, U., Codina, J. & Birnbaumer, L. J. biol. Chem. 265, 18576–18580 (1990).

    CAS  PubMed  Google Scholar 

  21. Strathmann, M., Wilke, T. M. & Simon, M. I. Proc. natn. Acad. Sci. U.S.A. 87, 6477–6481 (1990).

    Article  ADS  CAS  Google Scholar 

  22. Birnbaumer, L., Abramowitz, J. & Brown, A. Biochim. biophys. Acata 1031, 163–224 (1990).

    Article  CAS  Google Scholar 

  23. Masters, S. E., Strout, R. N. & Bourne, H. R. Protein Engng 1, 47–54 (1986).

    Article  CAS  Google Scholar 

  24. Collu, R. et al. Endocrinology 122, 1176–1178 (1988).

    Article  CAS  Google Scholar 

  25. Silbert, S., Michel, T., Lee, R. & Neer, E. J. J. biol. Chem. 265, 3102–3105 (1990).

    CAS  PubMed  Google Scholar 

  26. Izant, J. G. & Weintraub, H. Cell 36, 1007–1015 (1984).

    Article  CAS  Google Scholar 

  27. Weintraub, H., Izant, J. G. & Harland, R. M. Trends Genet. 1, 22–25 (1985).

    Article  CAS  Google Scholar 

  28. Green, P. J., Pines, O. & Inouye, M. A. Rev. Biochem. 55, 569–597 (1986).

    Article  CAS  Google Scholar 

  29. Shuttleworth, J. & Colman, A. EMBO J. 7, 427–434 (1988).

    Article  CAS  Google Scholar 

  30. Minshull, J. & Hunt, T. Nucleic Acids Res. 14, 6433–6451 (1986).

    Article  CAS  Google Scholar 

  31. Loke, S. L. et al. Proc. natn. Acad. Sci. U.S.A. 86, 3474–3478 (1989).

    Article  ADS  CAS  Google Scholar 

  32. Hélène, C. & Toulmé, J.-J. Biochim. biophys. Acta 1049, 99–125 (1990).

    Article  Google Scholar 

  33. White, R. E., Schonbrunn, A. & Armstrong, D. L. Nature 351, 570–573 (1991).

    Article  ADS  CAS  Google Scholar 

  34. Yatani, A. et al. Nature 336, 680–682 (1988).

    Article  ADS  CAS  Google Scholar 

  35. Mori, Y. et al. Nature 350, 398–402 (1991).

    Article  ADS  CAS  Google Scholar 

  36. Sunahara, R. K. et al. Nature 351, 614–619 (1991).

    Article  ADS  Google Scholar 

  37. Findlay, J. & Eliopoulos, E. Trends Pharmac. Sci. 11, 492–499 (1990).

    Article  CAS  Google Scholar 

  38. Changeux, J.-P. Trends Pharmac. Sci. 11, 485–492 (1990).

    Article  CAS  Google Scholar 

  39. Hsu, W. H. et al. J. biol. Chem. 265, 11220–11226 (1990).

    CAS  PubMed  Google Scholar 

  40. Nomenclature Committee of the International Union of Biochemistry Eur. J. Biochem. 150, 1–5 (1985).

  41. Offermanns, S., Schultz, G. & Rosenthal, W. Eur. J. Biochem. 180, 283–287 (1989).

    Article  CAS  Google Scholar 

  42. Jackson, D. A., Yuan, J. & Cook, P. R. J. Cell Sci. 90, 365–378 (1988).

    CAS  PubMed  Google Scholar 

  43. Graessmann, M. & Graessmann, A. Meth. Enzym. 101, 482–492 (1983).

    Article  CAS  Google Scholar 

  44. Dreyer, C., Scholz, E. & Hausen, P. Roux's Arch. dev. Biol. 191, 228–233 (1982).

    Article  CAS  Google Scholar 

  45. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. Pflügers. Arch. ges. Physiol. 391, 85–100 (1981).

    Article  CAS  Google Scholar 

  46. Tanabe, T. et al. Nature 315, 242–245 (1985).

    Article  ADS  CAS  Google Scholar 

  47. Jones, D. T. & Reed, R. R. J. biol. Chem. 262, 14241–14249 (1987).

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-W1000, Berlin, 33, Germany

    C. Kleuss, C. Ewel & B. Wittig

  2. Institut für Pharmakologie, Freie Universität Berlin, Arnimallee 22, D-W1000, Berlin, 33, Germany

    J. Hescheler, W. Rosenthal & G. Schultz

Authors
  1. C. Kleuss
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  2. J. Hescheler
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  3. C. Ewel
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  4. W. Rosenthal
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  5. G. Schultz
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  6. B. Wittig
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Kleuss, C., Hescheler, J., Ewel, C. et al. Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. Nature 353, 43–48 (1991). https://doi.org/10.1038/353043a0

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