64 Result(s)

Article

PCR-Based Ribosomal DNA Detection Technique for Microalga (Heterosigma carterae) Causing Red Tide and Its Application to a Biosensor Using Labeled Probe

A technique for detecting Raphidophycean, a bloom-forming genus of algae, was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the small subunit (SSU) ribo...

Ryoichi Asai, Kozue Ootani, Yoko Nomura, Chikashi Nakamura… in Marine Biotechnology (2003)

Article

Current research activity in biosensors

Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks [1] many biosensors and their associated techniques have been studied and dev...

Hideaki Nakamura, Isao Karube in Analytical and Bioanalytical Chemistry (2003)

Article

Fructose-2,6-bisphosphate contents were increased in response to salt, water and osmotic stress in leaves of Bruguiera gymnorrhiza by differential changes in the activity of the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase

The steady-state level of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphate 2-phosphatase (F6P2K/F26BPase) transcript has been found to be raised in the mangrove Bruguiera gymnorrhiza treated with 500 mM NaCl fo...

Toshiaki Banzai, Nobutaka Hanagata, Zvy Dubinsky, Isao Karube in Plant Molecular Biology (2003)

Article

Detection technique of asymmetric RT-PCR-based amplified single-stranded DNA and its application to biosensor for detection of mRNA for cyanobacteria, Anabaena variabilis

A real-time detection technique for mRNA was developed using a specific DNA probe. The design of the probe was based on a sequence polymorphism within the expressed mRNA of Anabaena variabilis PCC 7120 by using t...

Ryoichi Asai, Chikashi Nakamura, Kazunori Ikebukuro, Isao Karube… in Biotechnology Letters (2002)

Article

Molecular cloning and characterization of genes encoding BURP domain-containing protein in the mangrove, Bruguiera gymnorrhiza

We cloned and characterized four independent genes encoding BURP domain-containing proteins from mangrove, Bruguiera gymnorrhiza, two as cDNA (BgBDC2 and BgBDC3) and the other two as genomic DNA (S2 and L3). The...

Toshiaki Banzai, Kikuko Sumiya, Nobutaka Hanagata, Zvy Dubinsky, Isao Karube in Trees (2002)

Article

Molecular characterization and response to salt stress of mRNAs encoding cytosolic Cu/Zn superoxide dismutase and catalase from Bruguiera gymnorrhiza

To analyze the potential of the active oxygen-scavenging system of the cytosol in leaves of salt-stressed Bruguiera gymnorrhiza, we isolated a full-length cDNA encoding a 153-amino-acid sequence of cytosolic Cu/...

Taro Takemura, Nobutaka Hanagata, Zvy Dubinsky, Isao Karube in Trees (2002)

Article

Observation and Application of a Pattern in a DNA-Histone Mixture

Satoshi Sasaki, Isao Karube in Analytical Sciences (2001)

Article

Isolation of a bacterium from mangrove soil for degradation of sea sludge

Sea sludge, which is sediment of fish excrement and sewage on the sea bottom, continues to be a serious environmental problem. It has the potential to cause eutrophication and red tide, resulting in the death ...

Yoshihiro Ando, Naoya Mitsugi, Kazuyoshi Yano… in Applied Biochemistry and Biotechnology (2001)

Article

Sensor Chip Using a Plasma-polymerized Film for Surface Plasmon Resonance Biosensors: Reliable Analysis of Binding Kinetics

Hitoshi Muguruma, Ryohei Nagata, Runa Nakamura, Kimiharu Sato… in Analytical Sciences (2000)

Article

Multifunctional Biocompatible Membrane and Its Application to Fabricate A Miniaturized Glucose Sensor with Potential for Use In Vivo

A multifunctional membrane with biocompatibility, diffusion-limiting effect, and the ability to curtail the responses of an H₂O₂ electrode to ascorbate and urate was prepared. It was composed of MB, AB, and CTA, ...

Chien-Yuan Chen, Kazuhiko Ishihara, Nobuo Nakabayashi… in Biomedical Microdevices (1999)

Article

Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation

Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compoun...

Ritsuko Matsukawa, Kiyotaka Hatakeda, Shota Ito… in Applied Biochemistry and Biotechnology (1999)

Article

Purification of single stranded DNA from asymmetric PCR product using the SMART system

A method has been developed using the SMART system for the purification of single stranded DNA from a mixture containing single- and double-stranded DNA amplified using asymmetric PCR. The asymmetric PCR produ...

Eriko Kai, Koichi Sumikura, Kazunori Ikebukuro, Isao Karube in Biotechnology Techniques (1998)

Article

Enhanced eicosapentaenoic acid production by Navicula saprophila

Changes in the eicosapentaenoic acid (EPA) content of Navicula saprophila grown photoautotrophically and mixotrophically with the addition of acetate were examined in terms of their growth progress. Among four...

Makoto Kitano, Ritsuko Matsukawa, Isao Karube in Journal of Applied Phycology (1998)

Article

Changes in eicosapentaenoic acid content of Navicula saprophila, Rhodomonas salina and Nitzschia sp. under mixotrophic conditions

Three species of microalgae able to produce eicosapentaenoic acid(EPA) were collected from brackish and sea water around Japan. The species were identified as Navicula saprophila, Rhodomonassalina and Nitzschi...

Makoto Kitano, Ritsuko Matsukawa, Isao Karube in Journal of Applied Phycology (1997)

Article

Enzymatic screening of microalgae as a potential source of natural antioxidants

Extracts of over 100 species of microalgae both from nature and from laboratory cultures were screened for antioxidant activity. As an assay for antioxidant function, we examined the inhibition of the activity...

Ritsuko Matsukawa, Zvy Dubinsky, Kazuyoshi Masaki… in Applied Biochemistry and Biotechnology (1997)

Article

Photo-induced activation of cytochrome P450/reductase fusion enzyme coupled with spinach chloroplasts

Photoactivation of cytochrome P450 monooxygenase was studied using a combination of spinach chloroplasts and yeast microsomes containing rat P4501A1/yeast reductase fusion enzyme. Under illumination, in the re...

Young-Sug Kim, Masayuki Hara, Kazunori Ikebukuro, Jun Miyake… in Biotechnology Techniques (1996)

Article

Determination of cyanide using a microbial sensor

A microbial cyanide sensor was prepared, consisting of immobilizedSaccharomyces cerevisiae and an oxygen electrode. When the electrode was inserted into a solution containing glucose, the respiration activity of ...

Keijiro Nakanishi, Kazunori Ikebukuro… in Applied Biochemistry and Biotechnology (1996)

Article

A novel synthesis method for cyclodextrins from maltose in water-organic solvent systems

A novel enzymatic synthesis method of cyclodextrin (CD) from low-mol-wt maltose using cyclomaltodextrin glucanotransferase (CGTase) fromBacillus macerans has been developed in various water-organic solvent system...

Takashi Morita, Nobuyuki Yoshida, Isao Karube in Applied Biochemistry and Biotechnology (1996)