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Protein Purification by Reversed Phase Chromatography and Hydrophobic Interaction Chromatography
With the increasing analysis and studies on purified proteins and peptides with respect to their structure and function, the need for high resolution...
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Isotope-Dilution Liquid Chromatography–Tandem Mass Spectrometry for Detection of 5-Hydroxymethyluracil and 5-Formyluracil in DNA
5-Hydroxymethyluracil (5hmU) and 5-formyluracil (5fU) are mutagenic and major oxidized nucleobases in DNA that are derived from thymine (T) or...
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Chromatographic Separation and Quantitation of Sphingolipids from the Central Nervous System or Any Other Biological Tissue
Chromatographic separation and purification of an individual lipid to homogeneity have long been introduced. Using this concept, a more precise...
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Lectin and Liquid Chromatography-Based Methods for Immunoglobulin (G) Glycosylation Analysis
Immunoglobulin (Ig) glycosylation has been shown to dramatically affect its structure and effector functions. Ig glycosylation changes have been...
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Two-Dimensional Liquid Chromatography in Metabolomics and Lipidomics
Multidimensional separation systems have arisen in the last years to overcome certain limitations of the classical one-dimensional separations....
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Protein Purification by Ion Exchange Chromatography
Separation of similar biomolecules and proteins with little or no differences in molecular weight or without tags can be difficult with...
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Simultaneous and enantiospecific quantification of primaquine and carboxyprimaquine in human plasma using liquid chromatography-tandem mass spectrometry
BackgroundThe enantiomers of the 8-aminoquinoline anti-malarial primaquine have different pharmacological properties. Development of an analytical...
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Glioblastoma biomarkers in urinary extracellular vesicles reveal the potential for a ‘liquid gold’ biopsy
BackgroundBiomarkers that reflect glioblastoma tumour activity and treatment response are urgently needed to help guide clinical management,...
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Defining the reactivity of nanoparticles to peptides through direct peptide reactivity assay (DPRA) using a high pressure liquid chromatography system with a diode array detector
The possibility of inducing skin sensitization reactions following exposure to various chemicals can lead to skin diseases, and the evaluation of...
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Automated Multidimensional Nanoscale Chromatography for Ultrasensitive Targeted Mass Spectrometry
Recent advances in nanoscale separations and high-resolution mass spectrometry permit highly sensitive and accurate analyses of complex protein...
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Bioanalytical Assays: Gas Chromatography (GC)
The quantitative determination of analytes in biological matrices such as blood and urine is called bioanalysis. Bioanalysis regulatory authorities...
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Application of Design of Experiments® Approach-Driven Artificial Intelligence and Machine Learning for Systematic Optimization of Reverse Phase High Performance Liquid Chromatography Method to Analyze Simultaneously Two Drugs (Cyclosporin A and Etodolac) in Solution, Human Plasma, Nanocapsules, and Emulsions
The objectives of current investigation are (1) to find out wavelength of maximum absorbance (λ max ) for combined cyclosporin A and etodolac solution...
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Development, Quality by Design-Based Optimization, and Stability Assessment of Oral Liquid Formulations Containing Baclofen for Hospital Use
The absence of oral liquid pharmaceutical forms appropriate for use in pediatric and adult patients with difficulty swallowing is a public health...
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Liquid biopsy techniques and lung cancer: diagnosis, monitoring and evaluation
Lung cancer stands as the most prevalent form of cancer globally, posing a significant threat to human well-being. Due to the lack of effective and...
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Freeze-Dried Biopharmaceutical Formulations are Surprisingly Less Stable than Liquid Formulations during Drop**
PurposesThis article describes an interesting phenomenon in which optimized freeze-dried (FD) biopharmaceutical formulations are generally more prone...
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Liquid Biopsy in Adverse Neurodevelopment of Children: Problems and Prospects
Neurodevelopmental disorders in children have an important impact on the quality of life in the whole life cycle. Severe neurodevelopmental disorders...
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Cancer metabolites: promising biomarkers for cancer liquid biopsy
Cancer exerts a multitude of effects on metabolism, including the reprogramming of cellular metabolic pathways and alterations in metabolites that...
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Liquid biopsy techniques and pancreatic cancer: diagnosis, monitoring, and evaluation
Pancreatic cancer (PC) is one of the most common malignancies. Surgical resection is a potential curative approach for PC, but most patients are...
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A Static Headspace Gas Chromatography Method for Quantitation of Residual Solvents in Nanoformulations
Various organic solvents are widely used in the manufacturing, processing, and purification of drug substances, drug products, formulations,...
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Dried Urine Spots as a Sampling Approach for the Determination of Organophosphorus Insecticides by Gas Chromatography Tandem Mass Spectrometry
There are numerous methods described for the identification and quantification of organophosphorus insecticides, and these involve sample preparation...