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Article
Roxithromycin attenuates bleomycin-induced pulmonary fibrosis by targeting senescent cells
Idiopathic pulmonary fibrosis (IPF) is an aging‐associated disease with a poor prognosis. Emerging evidence has revealed that targeting senescent cells may be a potential treatment for IPF. In this study, we a...
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Protocol
PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a
Single-molecule real-time (SMRT) sequencing developed by Pacific BioSciences (PacBio) offers three major advantages compared to second-generation sequencing: long read length and high consensus accuracy, and a...
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Protocol
TSETA: A Third-Generation Sequencing-Based Computational Tool for Map** and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations
TSETA (Third-generation Sequencing to Enable Tetrad Analysis) is a fungus-centric software pipeline that utilizes chromosome-level sequence assembly for genome-wide and single-nucleotide-resolution map** of sin...
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Article
Genome-Wide Analysis of LAZ1 Gene Family from Maize
Lazarus 1 (LAZ1) is a six transmembrane protein with DUF300 domain, and functions as organic solute transporter in vertebrates. However, there was no any available literature on genome-wide identification of t...
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Article
Open AccessExpression response of chalcone synthase gene to inducing conditions and its effect on flavonoids accumulation in two medicinal species of Anoectochilus
Anoectochilus roxburghii and Anoectochilus formasanus are the major species of genus Anoectochilus used in traditional Chinese medicine for their abundant content of flavonoids and some other medicinal constituen...
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Article
Repeat-induced point (RIP) mutation in the industrial workhorse fungus Trichoderma reesei
Trichoderma reesei (syn. Hypocrea jecorina) is a filamentous ascomycete. Due to its capability of producing large amounts of lignocellulolytic enzymes and various heterologous proteins, this fungus has been widel...
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Article
Interaction network of core ABA signaling components in maize
We defined a comprehensive core ABA signaling network in monocot maize, including the gene expression, subcellular localization and interaction network of ZmPYLs, ZmPP2Cs, ZmSnRK2s and the puta...
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Article
A betaine aldehyde dehydrogenase gene from Ammopiptanthus nanus enhances tolerance of Arabidopsis to high salt and drought stresses
Glycine betaine functions as an osmotic protectant in response of plant to abiotic stresses. In higher plants, the betaine aldehyde dehydrogenase (BADH) catalyzes the key step of glycine betaine biosynthesis. ...
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Article
Open AccessTrichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
Trichoderma reesei (Ascomycota, Pezizomycotina) QM6a is a model fungus for a broad spectrum of physiological phenomena, including plant cell wall degradation, industrial production of ...
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Article
Cloning and characterization of vacuolar H+-pyrophosphatase gene (AnVP1) from Ammopiptanthus nanus and its heterologous expression enhances osmotic tolerance in yeast and Arabidopsis thaliana
Vacuolar H+-pyrophosphatase (H+-PPase) is one of the proton pumps involved in the response of plants to osmotic stress. In the present study, the vacuolar H+-PPase gene (AnVP1) was cloned from an epibiotic super-...
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Article
Phospholipase D family and its expression in response to abiotic stress in maize
Phospholipase D hydrolyzes phospholipids into phophatidic acid and choline, and plays important roles in growth, development, and stress response. Genome-wide identification for the PLD gene family has been ma...
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Article
Open AccessCandida albicans Dbf4-dependent Cdc7 kinase plays a novel role in the inhibition of hyphal development
Candida albicans is an opportunistic human fungal pathogen. The ability to switch among multiple cellular forms is key to its pathogenesis. The Dbf4-dependent protein kinase gene CDC7 is conserved due to its role...
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Chapter
Hybrid Infertility: The Dilemma or Opportunity of Applying Sexual Development to Improve Trichoderma reesei Industrial Strains
Trichoderma reesei (Teleomorph Hypocrea jecorina) RUT-C30 and QM9414 strains are the principal industrial producers of enzymes that hydrolyze lignocellulosic biomass into simple sugars, such as glucose and xylos...
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Article
Overexpression of a phospholipase Dα gene from Ammopiptanthus nanus enhances salt tolerance of phospholipase Dα1-deficient Arabidopsis mutant
A phospholipase Dα gene ( AnPLDα ) was cloned from xerophytic desert plant Ammopiptanthus nanus and its overexpression en...
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Article
Cloning and functional validation of molybdenum cofactor sulfurase gene from Ammopiptanthus nanus
The molybdenum cofactor sulfurase gene ( AnMCSU ) was cloned from xerophytic desert plant Ammopiptanthus nanus ...
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Article
Open AccessTrichoderma reesei meiosis generates segmentally aneuploid progeny with higher xylanase-producing capability
Hypocrea jecorina is the sexual form of the industrial workhorse fungus Trichoderma reesei that secretes cellulases and hemicellulases to degrade lignocellulosic biomass into simple sugars, suc...
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Article
Expression Profile of Maize MicroRNAs Corresponding to Their Target Genes Under Drought Stress
Microarray assay of four inbred lines was used to identify 303 microRNAs differentially expressed under drought stress. The microRNAs were used for bioinformatics prediction of their target genes. The majority...
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Article
RNA interference-mediated resistance to maize dwarf mosaic virus
Maize dwarf mosaic virus (MDMV) is a widespread pathogen that causes serious yield loss to maize crops. A hairpin RNA expression vector was constructed herein to overcome the low efficiency of cultural protect...
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Article
RNA Interference-Based Transgenic Maize Resistant to Maize Dwarf Mosaic Virus
Maize dwarf mosaic virus (MDMV) is a widespread pathogenic virus that causes serious loss of yield in maize (Zea mays). RNA interference (RNAi) triggered by hairpin RNA (hpRNA) transcribed from a transgenic inver...
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Article
Cloning and Characterization of Functional Trehalose-6-Phosphate Synthase Gene in Maize
Trehalose is a non-reducing disaccharide of glucose that functions as a compatible solute in the stabilization of biological structures under heat and desiccation stress in bacteria, fungi, and some “resurrect...