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RNA interference-mediated resistance to maize dwarf mosaic virus

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Abstract

Maize dwarf mosaic virus (MDMV) is a widespread pathogen that causes serious yield loss to maize crops. A hairpin RNA expression vector was constructed herein to overcome the low efficiency of cultural protection against MDMV and to improve the MDMV resistance mediated by a shorter transgenic inverted-repeat sequence. This expression vector contained a 451 bp inverted-repeat sequence, homologous to the protease gene (P1) of MDMV. It was used for the Agrobacterium tumefaciens-mediated transformation of maize calli induced from a susceptible inbred line. A total of 17 T2 transgenic lines were identified by both specific PCR amplification and Southern blot hybridization. Of these lines, 15 were evaluated for MDMV resistance in inoculation field trials under two environments. The relative replication levels of the P1 gene were analyzed by quantitative real-time (qRT)-PCR. Results demonstrated that all of the 15 T2 lines showed an enhanced resistance to MDMV in comparison with that of the non-transformed parent line. Six lines were deemed to be ‘resistant’ with an average disease index below 25 %, which was not significantly different from that of the resistant control. The relative replication levels of the virus gene were significantly reduced in these resistant T2 transgenic lines. The efficiency of virus gene silencing was directly related to the transgene copy numbers presented in these transgenic lines.

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Acknowledgments

The authors sincerely appreciate the financial support from the Projects of Development Plan of the State Key Fundamental Research (973 Project 2009CB118401), the National Key Science and Technology Special Project (2009ZX08003012B and 2013ZX08003-004), and the technical support from Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region. The authors thank the anonymous reviewers for their critical comments and suggestions, and Ms Dominique Thomas at Cornell University for her help in improving the English writing.

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  1. Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, Sichuan, People’s Republic of China

    Zhi-Yong Zhang, Ying-Ge Wang, **ao-Juan Shen, Lei Li, Shu-Feng Zhou, Wan-Chen Li & Feng-Ling Fu

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  1. Zhi-Yong Zhang
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  2. Ying-Ge Wang
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Correspondence to Wan-Chen Li or Feng-Ling Fu.

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Zhang, ZY., Wang, YG., Shen, XJ. et al. RNA interference-mediated resistance to maize dwarf mosaic virus. Plant Cell Tiss Organ Cult 113, 571–578 (2013). https://doi.org/10.1007/s11240-013-0289-z

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