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  1. No Access

    Article

    Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts

    Application of new genome editing technologies for plant improvement depends on efficient delivery of editing components into plant cells. For editing of canola, which is an important oil crop, CRISPR/Cas9 and Ag...

    Vladimir Sidorov, Dafu Wang, Ervin D. Nagy… in In Vitro Cellular & Developmental Biology … (2022)

  2. Article

    Open Access

    Demonstration of targeted crossovers in hybrid maize using CRISPR technology

    Naturally occurring chromosomal crossovers (CO) during meiosis are a key driver of genetic diversity. The ability to target CO at specific allelic loci in hybrid plants would provide an advantage to the plant ...

    Andrei Kouranov, Charles Armstrong, Ashok Shrawat… in Communications Biology (2022)

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  3. No Access

    Article

    “Cell grafting”: a new approach for transferring cytoplasmic or nuclear genome between plants

    A new method based on mixing and wounding of callus tissue was used to transfer plastid or nuclear DNA between cells.

    Vladimir Sidorov, Charles Armstrong, Thomas Ream, Xudong Ye… in Plant Cell Reports (2018)

  4. No Access

    Protocol

    Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus

    Transformation with Agrobacterium tumefaciens is the preferred method for delivery of transgenes into a wide range of plant species including maize. Optimized protocols for the Agrobacterium-mediated transformati...

    Vladimir Sidorov, David Duncan in Transgenic Maize (2009)

  5. No Access

    Article

    Agrobacterium-mediated transformation of seedling-derived maize callus

    Efficient production of seedling-derived Type I callus was demonstrated for several corn genotypes including commercial inbred lines. Seeds were germinated on MS-based medium containing 10 mg l−1 picloram and 3 m...

    Vladimir Sidorov, Larry Gilbertson, Prince Addae, David Duncan in Plant Cell Reports (2006)

  6. No Access

    Article

    Complementation in somatic hybrids indicates four types of nitrate reductase deficient lines in Nicotiana plumbaginifolia

    Allelism of nine nitrate reductase deficient (NR−) Nicotiana plumbaginifolia cell lines was tested by complementation after protoplast fusion. Complementation was recognized by the appearance of somatic hybrid co...

    László Márton, Vladimir Sidorov, Grazia Biasini… in Molecular and General Genetics MGG (1982)