-
Article
Open AccessReconstitution of a telomeric replicon organized by CST
Telomeres, the natural ends of linear chromosomes, comprise repeat-sequence DNA and associated proteins1. Replication of telomeres allows continued proliferation of human stem cells and immortality of cancer cell...
-
Article
Open AccessPublisher Correction: Nuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention
-
Article
Open AccessNuclear compartmentalization of TERT mRNA and TUG1 lncRNA is driven by intron retention
The spatial partitioning of the transcriptome in the cell is an important form of gene-expression regulation. Here, we address how intron retention influences the spatio-temporal dynamics of transcripts from t...
-
Article
Publisher Correction: Sha** human telomeres: from shelterin and CST complexes to telomeric chromatin organization
A Correction to this paper has been published: https://doi.org/10.1038/s41580-021-00353-x.
-
Article
Sha** human telomeres: from shelterin and CST complexes to telomeric chromatin organization
The regulation of telomere length in mammals is crucial for chromosome end-cap** and thus for maintaining genome stability and cellular lifespan. This process requires coordination between telomeric protein ...
-
Article
RNA is essential for PRC2 chromatin occupancy and function in human pluripotent stem cells
Many chromatin-binding proteins and protein complexes that regulate transcription also bind RNA. One of these, Polycomb repressive complex 2 (PRC2), deposits the H3K27me3 mark of facultative heterochromatin an...
-
Article
Molecular analysis of PRC2 recruitment to DNA in chromatin and its inhibition by RNA
Biochemical reconstitution of PRC2 interactions with chromatinized templates demonstrates that protein-free linker DNA dominates the PRC2-nucleosome interaction, while RNA inhibits binding.
-
Article
Open AccessReconstitution of human shelterin complexes reveals unexpected stoichiometry and dual pathways to enhance telomerase processivity
The human shelterin proteins associate with telomeric DNA to confer telomere protection and length regulation. They are thought to form higher-order protein complexes for their functions, but studies of shelte...
-
Article
Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects
New evidence that human telomerase RNA (hTR) degradation by EXOSC10 or DCP2 and XRN1 reduces telomerase activity when dyskerin is compromised suggests that RNA decay pathways may provide future therapeutic tar...
-
Article
Open AccessA novel two-step genome editing strategy with CRISPR-Cas9 provides new insights into telomerase action and TERT gene expression
To facilitate indefinite proliferation, stem cells and most cancer cells require the activity of telomerase, which counteracts the successive shortening of telomeres caused by incomplete DNA replication at the...
-
Article
Protein-RNA interaction restricts telomerase from running through the stop sign
Telomerase is a unique reverse transcriptase in that it repetitively uses a short piece of its RNA component as template to synthesize DNA. A new crystal structure of a part of the Tetrahymena telomerase ribonucl...
-
Article
Academia and industry: Companies on campus
Housing industry labs in academic settings benefits all parties, say Jana J. Watson-Capps and Thomas R. Cech.
-
Article
Promiscuous RNA binding by Polycomb repressive complex 2
Polycomb repressive complex 2 (PRC2) is a histone methyltransferase required for epigenetic silencing during development and cancer, and it can be recruited to chromatin by long noncoding RNAs. In vitro binding s...
-
Article
Open Access3′ terminal diversity of MRP RNA and other human noncoding RNAs revealed by deep sequencing
Post-transcriptional 3′ end processing is a key component of RNA regulation. The abundant and essential RNA subunit of RNase MRP has been proposed to function in three distinct cellular compartments and theref...
-
Article
Finding the end: recruitment of telomerase to telomeres
The ends of linear chromosomes pose two problems to eukaryotic cells: the end-protection problem; and the end-replication problem. End protection is necessary ...
-
Article
The TEL patch of telomere protein TPP1 mediates telomerase recruitment and processivity
Using separation-of-function mutations of TPP1 that inhibit telomerase binding while maintaining telomere cap**, a region on the surface of TPP1, the TEL patch, is identified and found to be required for bot...
-
Article
The RNA accordion model for template positioning by telomerase RNA during telomeric DNA synthesis
Telomerase uses its associated RNA as a template for processive addition of telomeric DNA repeats. Biochemistry and smFRET analysis are now used to investigate how the RNA template moves along the active site,...
-
Article
Telomere shortening and loss of self-renewal in dyskeratosis congenita induced pluripotent stem cells
Dyskeratosis congenita, a disease characterized by defective maintenance of blood, pulmonary and epidermal tissues, is caused by defects in genes required for telomere homeostasis. Short telomeres are thought ...
-
Article
A natural ribozyme with 3′,5′ RNA ligase activity
-
Article
Mutation in TERT separates processivity from anchor-site function
Repeat-addition processivity (RAP), that is, generating multiple DNA repeats from a single template without primer dissociation, is a key property of telomerase. In the Tetrahymena reverse-transcriptase component...
