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Protein-RNA interaction restricts telomerase from running through the stop sign

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Telomerase is a unique reverse transcriptase in that it repetitively uses a short piece of its RNA component as template to synthesize DNA. A new crystal structure of a part of the Tetrahymena telomerase ribonucleoprotein reveals how reverse transcription is limited to this specific template region.

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Figure 1: Hypothesized evolutionary path of biocatalysis.
Figure 2: Comparison between telomerase and other reverse transcriptases.
Figure 3: Location of the TBE in TER and the TRBD in the TERT protein.

References

  1. Greider, C.W. & Blackburn, E.H. Nature 337, 331–337 (1989).

    Article  CAS  Google Scholar 

  2. Lingner, J. et al. Science 276, 561–567 (1997).

    Article  CAS  Google Scholar 

  3. Tzfati, Y., Fulton, T.B., Roy, J. & Blackburn, E.H. Science 288, 863–867 (2000).

    Article  CAS  Google Scholar 

  4. Lai, C.K., Miller, M.C. & Collins, K. Genes Dev. 16, 415–420 (2002).

    Article  CAS  Google Scholar 

  5. Chen, J.L. & Greider, C.W. Genes Dev. 17, 2747–2752 (2003).

    Article  CAS  Google Scholar 

  6. Box, J.A., Bunch, J.T., Zappulla, D.C., Glynn, E.F. & Baumann, P. J. Biol. Chem. 283, 24224–24233 (2008).

    Article  CAS  Google Scholar 

  7. Jansson, L.I. et al. Nat. Struct. Mol. Biol. 22, 883–888 (2015).

    Article  CAS  Google Scholar 

  8. Meyerson, M. J. Clin. Oncol. 18, 2626–2634 (2000).

    Article  CAS  Google Scholar 

  9. White, H.B. III. in The Pyridine Nucleotide Coenzymes (eds. Everse, J., Anderson, B. & You, K.-S.) 1–17 (Academic Press, New York, 1982).

    Book  Google Scholar 

  10. Miller, M.C. & Collins, K. Proc. Natl. Acad. Sci. USA 99, 6585–6590 (2002).

    Article  CAS  Google Scholar 

  11. Qiao, F. & Cech, T.R. Nat. Struct. Mol. Biol. 15, 634–640 (2008).

    Article  CAS  Google Scholar 

  12. Jacobs, S.A., Podell, E.R. & Cech, T.R. Nat. Struct. Mol. Biol. 13, 218–225 (2006).

    Article  CAS  Google Scholar 

  13. Rouda, S. & Skordalakes, E. Structure 15, 1403–1412 (2007).

    Article  CAS  Google Scholar 

  14. Mitchell, M., Gillis, A., Futahashi, M., Fujiwara, H. & Skordalakes, E. Nat. Struct. Mol. Biol. 17, 513–518 (2010).

    Article  CAS  Google Scholar 

  15. Jiang, J. et al. Nature 496, 187–192 (2013).

    Article  CAS  Google Scholar 

  16. Harkisheimer, M., Mason, M., Shuvaeva, E. & Skordalakes, E. Structure 21, 1870–1878 (2013).

    Article  CAS  Google Scholar 

  17. Huang, J. et al. Nat. Struct. Mol. Biol. 21, 507–512 (2014).

    Article  CAS  Google Scholar 

  18. Greider, C.W. Mol. Cell. Biol. 11, 4572–4580 (1991).

    Article  CAS  Google Scholar 

  19. Lue, N.F. BioEssays 26, 955–962 (2004).

    Article  CAS  Google Scholar 

  20. Nakamura, T.M. et al. Science 277, 955–959 (1997).

    Article  CAS  Google Scholar 

  21. Bryan, T.M., Goodrich, K.J. & Cech, T.R. Mol. Cell 6, 493–499 (2000).

    Article  CAS  Google Scholar 

  22. Miller, M.C., Liu, J.K. & Collins, K. EMBO J. 19, 4412–4422 (2000).

    Article  CAS  Google Scholar 

  23. Berman, A.J., Akiyama, B.M., Stone, M.D. & Cech, T.R. Nat. Struct. Mol. Biol. 18, 1371–1375 (2011).

    Article  CAS  Google Scholar 

  24. Wu, R.A. & Collins, K. EMBO J. 33, 921–935 (2014).

    Article  CAS  Google Scholar 

  25. Akiyama, B.M., Gomez, A. & Stone, M.D. J. Biol. Chem. 288, 22141–22149 (2013).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Linghe ** and Thomas R. Cech are at the Department of Molecular, Cellular and Developmental Biology, BioFrontiers Institute, and the Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado, USA.,

    Linghe ** & Thomas R Cech

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  1. Linghe **
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  2. Thomas R Cech
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Correspondence to Thomas R Cech.

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Competing interests

Thomas R. Cech is on the Board of Directors of Merck, Inc.

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**, L., Cech, T. Protein-RNA interaction restricts telomerase from running through the stop sign. Nat Struct Mol Biol 22, 835–836 (2015). https://doi.org/10.1038/nsmb.3118

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