15 Result(s)
within
Sushila Maharjan
English
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Article
Open AccessVolumetric additive manufacturing of pristine silk-based (bio)inks
Volumetric additive manufacturing (VAM) enables fast photopolymerization of three-dimensional constructs by illuminating dynamically evolving light patterns in the entire build volume. However, the lack of bio...
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Protocol
Microfluidic Coaxial Bioprinting of Hollow, Standalone, and Perfusable Vascular Conduits
Three-dimensional bioprinting represents promising approach for fabricating standalone and perfusable vascular conduits using biocompatible materials. Here we describe a step-by-step method by using a multicha...
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Article
3D human nonalcoholic hepatic steatosis and fibrosis models
This study presents a simple and robust three-dimensional human hepatic tissue model to emulate steatotic and fibrotic conditions and provide an in vitro model for drug testing and mechanistic studies. Using a...
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Article
Open AccessEngineering multifunctional bactericidal nanofibers for abdominal hernia repair
The engineering of multifunctional surgical bactericidal nanofibers with inherent suitable mechanical and biological properties, through facile and cheap fabrication technology, is a great challenge. Moreover,...
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Article
Open AccessCirculatory shear stress induces molecular changes and side population enrichment in primary tumor-derived lung cancer cells with higher metastatic potential
Cancer is a leading cause of death and disease worldwide. However, while the survival for patients with primary cancers is improving, the ability to prevent metastatic cancer has not. Once patients develop met...
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Article
A hepatocellular carcinoma–bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles
We report the development of a metastasis-on-a-chip platform to model and track hepatocellular carcinoma (HCC)–bone metastasis and to analyze the inhibitory effect of an herb-based compound, thymoquinone (TQ),...
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Article
Open AccessA new way of producing pediocin in Pediococcus acidilactici through intracellular stimulation by internalized inulin nanoparticles
One of the most challenging aspects of probiotics as a replacement for antibiotics is to enhance their antimicrobial activity against pathogens. Given that prebiotics stimulate the growth and/or activity of pr...
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Article
Local Delivery of CTGF siRNA with Poly(sorbitol-co-PEI) Reduces Scar Contraction in Cutaneous Wound Healing
Healing process in scarring inevitably produces a considerable amount of non-organized dense collagen-rich matrix called scar thus impairing the native structure of skin. Connective tissue growth factor (CTGF)...
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Article
Gene therapy for bone tissue engineering
Gene therapy holds a great promise and has been extensively investigated to improve bone formation and regeneration therapies in bone tissue engineering. A variety of osteogenic genes can be delivered by combi...
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Article
Open AccessSoluble RANKL expression in Lactococcus lactis and investigation of its potential as an oral vaccine adjuvant
To initiate mucosal immune responses, antigens in the intestinal lumen must be transported into gut-associated lymphoid tissue through M cells. Recently, it has been increasingly recognized that receptor activ...
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Chapter
Marine Materials: Gene Delivery
The hurdle in the gene therapy is a lack of suitable gene carrier although animal virus vectors have been mostly used in vivo and in clinical applications due to their high transfection efficiency. However, th...
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Article
Metabolic Engineering of Nocardia sp. CS682 for Enhanced Production of Nargenicin A1
A number of secondary metabolites having therapeutic importance have been reported from the genus Nocardia. One of the polyketide antibiotic compounds isolated from Nocardia is nargenicin A1. Recently, nargenicin...
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Article
Biosynthesis of the nargenicin A1 pyrrole moiety from Nocardia sp. CS682
A number of structurally diverse natural products harboring pyrrole moieties possess a wide range of biological activities. Studies on biosynthesis of pyrrole ring have shown that pyrrole moieties are derived ...
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Article
Metabolic engineering of Streptomyces venezuelae for malonyl-CoA biosynthesis to enhance heterologous production of polyketides
Using metabolic engineering, we developed Streptomyces venezuelae YJ028 as an efficient heterologous host to increase the malonyl-CoA pool to be directed towards enhanced production of various polyketides. To pro...
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Article
Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin
Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae ATCC 15439, to produce 14-membered pikromycin antibiotics. The production of pikrom...
