Summary
Cell lines resistant to 50 μg ml-1 kanamycin sulphate were isolated from cell suspension cultures initiated from a haploid Nicotiana sylvestris plant. One line, KR103, has been studied in detail. Resistance of this line was shown to be stable in the absence of the drug. KR103 was found also to be resistant to streptomycin, another inhibitor of 70S ribosomal protein synthesis.
Both KR103 and the sensitive line convert kanamycin, but not streptomycin, to a form which is no longer effective in a bacterial bioassay, while maintaining its toxicity for sensitive plant cells.
KR103 is defective in morphogenesis and plastid development.
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References
Benveniste, R., Davies, J.: Mechanisms oif antibiotic resistance in bacteria. Ann. Rev. Biochem. 42, 471–506 (1973)
Binding, H.: Selektion in Kalluskulturen mit haploiden Zellen. Z. Pflanzenzüchtg. 67, 33–38 (1972)
Binding, H., Binding, K., Straub, J.: Selektion in Gewebekulturen mit Haploiden. Naturwissenschaften 57, 138–139 (1970)
Dix, P.J., Street, H.E.: Effect of p-fluorophenylalanine (PFP) on the growth of cell lines differing in ploidy and derived from Nicotiana sylvestris. Plant Sci. Lett. 3, 283–288 (1974)
Henshaw, C.G., Jha, K.K., Mehta, A.R., Shakeshaft, D.J., Street, H.E.: Studies on the growth in culture of plant cells. I. Growth pattern in batch propagated suspension cultures. J. exp. Bot. 17, 562–577 (1966)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965)
Kubitschek, H.E., Venema, G.: Multiple mutations induced in Bacillus subtilis by ultraviolet light. Mutation Res. 35, 325–328 (1976)
Linsmaier, E.M., Skoog, E.: Organic growth factor requirements of tobacco tissue culture. Physiol. Plantarum 18, 100–127 (1965)
Maliga, P.: Isolation of mutants of cultured plant cells. In: Cell genetics in higher plants (eds. D. Dudits, G.L. Farkas, P. Maliga), pp. 59–77 Budapest: Publishing House of the Hungarian Academy of Sciences 1976
Maliga, P., Dix, P.J., Márton, L., Lázár, G.: Tissue culture as a tool for the isolation of mutants in flowering plants. In: Proc. of the Symposium on the use of plant tissue culture in breeding. Olomouc, 6–11 September, 1976 (in the press)
Maliga, P., Lázár, G., Joó, F., H.-Nagy, A., Menczel, L.: Restoration of morphogenic potential in Nicotiana by somatic hybridisation. Molec. gen. Genet. 157, 291–296 (1977)
Maliga, P., Lázár, G., Sváb, Z., Nagy, F.: Transient cycloheximide resistance in a tobacco cell line. Molec. gen. Genet. 149, 267–271 (1976)
Maliga, P., Sz.-Breznovits, Á., Márton, L.: Streptomycin-resistant plants from callus cultures of haploid tobacco. Nature (Lond.) New Biol. 244, 401–403 (1973)
Melchers, G.: Haploid higher plants for plant breeding. Z. Pflanzenzüchtg. 67, 19–32 (1972)
Melchers, G.: Microbial techniques in somatic hybridization by fusion of protoplasts. Somatic plant hybridization by fusion of protoplasts (ed. Brinkley and Porter). International Cell Biology 1976–1977, p. 207–215. New York: Rockefeller University Press 1977
Melchers, G., Labib, G.: Somatic hybridisation of plants by fusion of protoplasts. Selection of Light Resistant Hybrids oif “Haploid” Light Sensitive Varieties of Tobacco. Molec. gen. Genet. 135, 277–294 (1974)
Melchers, G., Sacristán, M.D.: Somatic hybridization of plants by fusion of protoplasts. II. The chromosome numbers of somatic hybrid plants of 4 different fusion experiments. In: La culture des tissus et des cellules des végétaux. Paris-New York-Barcelona-Milán: Masson 1977
Milloning, G.: Advantage of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physics 32, 1637 (1961)
Nagy, J.I., Maliga, P.: Callus induction and plant regeneration from mesophyll protoplasts of Nicotiana sylvestris. Z. Pflanzenphysiol. 78, 453–455 (1976)
Nitsch, J.P., Nitsch, C.: Haploid plants from pollen grains. Science 163, 85–86 (1969)
Orosz, L., Sik, T.: Genetic map** of Rhizobiophage 16-3. Acta microbiol. Acad. Sci. hung. 17, 185–194 (1970)
Reynolds, E.S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol. 17, 208 (1963)
Smith, H.H., Kao, K.N., Combatti, N.C.: Interspecific hybridisation by protoplast fusion in Nicotiana. J. Hered. 67, 123–128 (1976)
Street, H.E.: Plant cell cultures: present and projected applications for studies in genetics. In: Genetic manipulations with plant material (ed. J. Ledoux), pp. 231–244. New York-London: Plenum Press 1975
Sung, Z.R.: Mutagenesis of cultured plant cells. Genetics 84, 51–57 (1976)
Umiel, N., Goldner, R.: Effects of streptomycin on diploid tobacco callus cultures and the isolation of resistant mutants. Protoplasma 89, 83–89 (1976)
Widholm, J.M.: Selection and characterisation of biochemical mutants. In: Plant tissue culture and its bio-technological applications (eds. W. Barz, E. Reinhard, M.H. Zenk), pp. 112–122. Berlin-Heidelberg-New York: Springer 1977
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Dix, P.J., Joó, F. & Maliga, P. A cell line of Nicotiana sylvestris with resistance to kanamycin and streptomycin. Molec. Gen. Genet. 157, 285–290 (1977). https://doi.org/10.1007/BF00268664
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DOI: https://doi.org/10.1007/BF00268664