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Open AccessCOX17 acetylation via MOF–KANSL complex promotes mitochondrial integrity and function
Reversible acetylation of mitochondrial proteins is a regulatory mechanism central to adaptive metabolic responses. Yet, how such functionally relevant protein acetylation is achieved remains unexplored. Here ...
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Article
Author Correction: A multipoint guidance mechanism for β-barrel folding on the SAM complex
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Article
A multipoint guidance mechanism for β-barrel folding on the SAM complex
Mitochondrial β-barrel proteins are essential for the transport of metabolites, ions and proteins. The sorting and assembly machinery (SAM) mediates their folding and membrane insertion. We report the cryo-ele...
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Article
Open AccessMitochondrial complexome reveals quality-control pathways of protein import
Mitochondria have crucial roles in cellular energetics, metabolism, signalling and quality control1–4. They contain around 1,000 different proteins that often assemble into complexes and supercomplexes such as re...
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Article
Author Correction: Mitochondrial proteins: from biogenesis to functional networks
A Correction to this paper has been published: https://doi.org/10.1038/s41580-021-00361-x.
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Article
Mitochondrial sorting and assembly machinery operates by β-barrel switching
The mitochondrial outer membrane contains so-called β-barrel proteins, which allow communication between the cytosol and the mitochondrial interior1–3. Insertion of β-barrel proteins into the outer membrane is me...
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Article
Open AccessThe mitochondrial carrier pathway transports non-canonical substrates with an odd number of transmembrane segments
The mitochondrial pyruvate carrier (MPC) plays a central role in energy metabolism by transporting pyruvate across the inner mitochondrial membrane. Its heterodimeric composition and homology to SWEET and semi...
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Article
Structure of the mitochondrial import gate reveals distinct preprotein paths
The translocase of the outer mitochondrial membrane (TOM) is the main entry gate for proteins1–4. Here we use cryo-electron microscopy to report the structure of the yeast TOM core complex5–9 at 3.8-Å resolution....
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Article
Mitochondrial proteins: from biogenesis to functional networks
Mitochondria are essential for the viability of eukaryotic cells as they perform crucial functions in bioenergetics, metabolism and signalling and have been associated with numerous diseases. Recent functional...
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Article
Open AccessRegulated membrane remodeling by Mic60 controls formation of mitochondrial crista junctions
The mitochondrial contact site and cristae organizing system (MICOS) is crucial for the formation of crista junctions and mitochondrial inner membrane architecture. MICOS contains two core components. Mic10 sh...
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Article
Open AccessSeparating mitochondrial protein assembly and endoplasmic reticulum tethering by selective coupling of Mdm10
The endoplasmic reticulum–mitochondria encounter structure (ERMES) connects the mitochondrial outer membrane with the ER. Multiple functions have been linked to ERMES, including maintenance of mitochondrial mo...
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Article
Open AccessVisualizing active membrane protein complexes by electron cryotomography
Unravelling the structural organization of membrane protein machines in their active state and native lipid environment is a major challenge in modern cell biology research. Here we develop the STAMP (Specific...
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Article
Mitochondrial inner membrane protease promotes assembly of presequence translocase by removing a carboxy-terminal targeting sequence
The presequence translocase of the inner mitochondrial membrane (TIM23 complex) is essential for importing cleavable preproteins into mitochondria. The preproteins contain amino-terminal targeting sequences th...
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Article
Voltage-coupled conformational dynamics of mitochondrial protein-import channel
Mitochondria contain multisubunit translocases to import preproteins from the cytosol. The presequence translocase of the inner membrane operates in a voltage-gated manner, but how a preprotein-conducting chan...
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Mitochondriale Systeme für den Import von Proteinen
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Mitochondrial protein import: from proteomics to functional mechanisms
Proteomic studies revealed that mitochondria contain ∼1,000 (in yeast) to 1,500 (in humans) different proteins and led to the recent identification of new mito...
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Protocol
Native Techniques for Analysis of Mitochondrial Protein Import
During the evolution of eukaryotic cells, the majority of mitochondrial genes have been transferred to the nuclear genome with the consequence that most mitochondrial proteins have to be imported into the orga...
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Article
Motor-free mitochondrial presequence translocase drives membrane integration of preproteins
The mitochondrial inner membrane is the central energy-converting membrane of eukaryotic cells. The electrochemical proton gradient generated by the respiratory chain drives the ATP synthase. To maintain this ...
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Protocol
The Mitochondrial Machinery for Import of Precursor Proteins
Mitochondria contain a small genome that codes for approx 1% of the total number of proteins that reside in the mitochondria. Hence, most mitochondrial proteins are encoded for by the nuclear genome. After tra...
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Protocol
Isolation of Yeast Mitochondria
Often preparations of isolated organelles contain other, unwanted, cellular components. For biochemical experiments to determine the localization of newly identified proteins, or to determine the whole set of ...
