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Article
Systems-level effects of allosteric perturbations to a model molecular switch
Molecular switch proteins whose cycling between states is controlled by opposing regulators1,2 are central to biological signal transduction. As switch proteins function within highly connected interaction networ...
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Article
A 31-residue peptide induces aggregation of tau's microtubule-binding region in cells
The self-propagation of misfolded conformations of tau underlies neurodegenerative diseases, including Alzheimer's. There is considerable interest in discovering the minimal sequence and active conformational ...
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Article
Histone demethylase KDM5A is regulated by its reader domain through a positive-feedback mechanism
The retinoblastoma binding protein KDM5A removes methyl marks from lysine 4 of histone H3 (H3K4). Misregulation of KDM5A contributes to the pathogenesis of lung and gastric cancers. In addition to its catalyti...
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Protocol
Biophysical Methods for Identifying Fragment-Based Inhibitors of Protein-Protein Interactions
Fragment-based lead discovery complements high-throughput screening and computer-aided drug design for the discovery of small-molecule inhibitors of protein-protein interactions. Fragments are molecules with m...
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Article
Open Access1H nuclear magnetic resonance brain metabolomics in neonatal mice after hypoxia–ischemia distinguished normothermic recovery from mild hypothermia recoveries
Mild brain hypothermia (31–34 °C) after neonatal hypoxia–ischemia (HI) improves neurodevelopmental outcomes in human and animal neonates. Using an asphyxia model with neonatal mice treated with mild hypothermi...
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Article
The structural basis of yeast prion strain variants
A comprehensive structural analysis of the complete and unmodified Sup35 prion domain in two distinct infectious conformations is presented. A variety of techniques is used to provide structural details of the...
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Article
Application of amino acid type-specific 1H- and 14N-labeling in a 2H-, 15N-labeled background to a 47 kDa homodimer: Potential for NMR structure determination of large proteins
NMR investigations of larger macromolecules (>20 kDa) are severely hindered by rapid 1H and 13C transverse relaxation. Replacement of non-exchangeable protons with deuterium removes many efficient 1H-1H and 1H...