18 Result(s)
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Marc R. Friedländer
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How ancient RNA survives and what we can learn from it
Although normally transient, RNA can persist postmortem when preserved by cold, desiccation or chemical treatment. In this Comment, we discuss how ancient RNA enables the study of gene expression of (pre)histo...
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Open AccessDetection of transcriptome-wide microRNA–target interactions in single cells with agoTRIBE
MicroRNAs (miRNAs) exert their gene regulatory effects on numerous biological processes based on their selection of target transcripts. Current experimental methods available to identify miRNA targets are labo...
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Open AccessThe SWI/SNF subunit BRG1 affects alternative splicing by changing RNA binding factor interactions with nascent RNA
BRG1 and BRM are ATPase core subunits of the human SWI/SNF chromatin remodelling complexes mainly associated with transcriptional initiation. They also have a role in alternative splicing, which has been shown...
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Open AccessA combined approach for single-cell mRNA and intracellular protein expression analysis
Combined measurements of mRNA and protein expression in single cells enable in-depth analysis of cellular states. We present SPARC, an approach that combines single-cell RNA-sequencing with proximity extension...
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Open AccessNuclear gene proximity and protein interactions shape transcript covariations in mammalian single cells
Single-cell RNA sequencing studies on gene co-expression patterns could yield important regulatory and functional insights, but have so far been limited by the confounding effects of differentiation and cell c...
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Male swarming aggregation pheromones increase female attraction and mating success among multiple African malaria vector mosquito species
Accumulating behavioural data indicate that aggregation pheromones may mediate the formation and maintenance of mosquito swarms. However, chemical cues possibly luring mosquitoes to swarms have not been adequa...
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Open AccessEXOSC10 is required for RPA assembly and controlled DNA end resection at DNA double-strand breaks
The exosome is a ribonucleolytic complex that plays important roles in RNA metabolism. Here we show that the exosome is necessary for the repair of DNA double-strand breaks (DSBs) in human cells and that RNA c...
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Open AccessmiRTrace reveals the organismal origins of microRNA sequencing data
We present here miRTrace, the first algorithm to trace microRNA sequencing data back to their taxonomic origins. This is a challenge with profound implications for forensics, parasitology, food control, and re...
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Open AccessSignatures of positive selection reveal a universal role of chromatin modifiers as cancer driver genes
Tumors are composed of an evolving population of cells subjected to tissue-specific selection, which fuels tumor heterogeneity and ultimately complicates cancer driver gene identification. Here, we integrate c...
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Open AccessSequence variation between 462 human individuals fine-tunes functional sites of RNA processing
Recent advances in the cost-efficiency of sequencing technologies enabled the combined DNA- and RNA-sequencing of human individuals at the population-scale, making genome-wide investigations of the inter-indiv...
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Open AccessCharacterisation of novel microRNAs in the Black flying fox (Pteropus alecto) by deep sequencing
Bats are a major source of new and emerging viral diseases. Despite the fact that bats carry and shed highly pathogenic viruses including Ebola, Nipah and SARS, they rarely display clinical symptoms of infecti...
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Open AccessEvidence for the biogenesis of more than 1,000 novel human microRNAs
MicroRNAs (miRNAs) are established regulators of development, cell identity and disease. Although nearly two thousand human miRNA genes are known and new ones are continuously discovered, no attempt has been m...
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Correction: Corrigendum: Reproducibility of high-throughput mRNA and small RNA sequencing across laboratories
Nat. Biotechnol. 31, 1015–1022 (2013); doi:10.1038/nbt.2702; published online 15 September 2013; corrected after print 8 November 2013 In the version of this article initially published, in the list of members...
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Reproducibility of high-throughput mRNA and small RNA sequencing across laboratories
RNA sequencing of 465 human lymphoblastoid cell lines across seven European laboratories shows the feasibility of transcriptome sequencing for population-wide and cross-biobank studies.
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Transcriptome and genome sequencing uncovers functional variation in humans
Genome sequencing projects are discovering millions of genetic variants in humans, and interpretation of their functional effects is essential for understanding the genetic basis of variation in human traits. ...
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Open AccessDeciphering the porcine intestinal microRNA transcriptome
While more than 700 microRNAs (miRNAs) are known in human, a comparably low number has been identified in swine. Because of the close phylogenetic distance to humans, pigs serve as a suitable model for studyin...
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Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression
Fluorescence-activated cell sorting of worm embryos promises to replace manual sorting of staged embryos and yields large populations highly enriched in specific developmental stages, allowing high-throughput ...
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Discovering microRNAs from deep sequencing data using miRDeep
The capacity of highly parallel sequencing technologies to detect small RNAs at unprecedented depth suggests their value in systematically identifying microRNAs (miRNAs). However, the identification of miRNAs ...
