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Article
Open AccessTissue-specific RNA Polymerase II promoter-proximal pause release and burst kinetics in a Drosophila embryonic patterning network
Formation of tissue-specific transcriptional programs underlies multicellular development, including dorsoventral (DV) patterning of the Drosophila embryo. This involves interactions between transcriptional enhan...
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Article
Open AccessGenetic atlas of hygro-and thermosensory cells in the vinegar fly Drosophila melanogaster
The ability of animals to perceive and respond to sensory information is essential for their survival in diverse environments. While much progress has been made in understanding various sensory modalities, the...
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Article
Open AccessA combined approach for single-cell mRNA and intracellular protein expression analysis
Combined measurements of mRNA and protein expression in single cells enable in-depth analysis of cellular states. We present SPARC, an approach that combines single-cell RNA-sequencing with proximity extension...
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Article
Spatially resolved transcriptome profiling in model plant species
Understanding complex biological systems requires functional characterization of specialized tissue domains. However, existing strategies for generating and analysing high-throughput spatial expression profile...
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Article
Open AccessEvolutionary history of host use, rather than plant phylogeny, determines gene expression in a generalist butterfly
Although most insect species are specialized on one or few groups of plants, there are phytophagous insects that seem to use virtually any kind of plant as food. Understanding the nature of this ability to fee...
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Protocol
Identification and Verification of microRNAs by High-Throughput Sequencing
High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and prefere...
