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  1. Article

    Open Access

    Unraveling the genome of Bacillus velezensis MEP218, a strain producing fengycin homologs with broad antibacterial activity: comprehensive comparative genome analysis

    Bacillus sp. MEP218, a soil bacterium with high potential as a source of bioactive molecules, produces mostly C16–C17 fengycin and other cyclic lipopeptides (CLP) when growing under previously optimized culture c...

    Daniela Medeot, Analía Sannazzaro, María Julia Estrella in Scientific Reports (2023)

  2. Article

    Open Access

    Diversity of gene expression responses to light quality in barley

    Light quality influence on barley development is poorly understood. We exposed three barley genotypes with either sensitive or insensitive response to two light sources producing different light spectra, fluor...

    Álvaro Rodríguez del Río, Arantxa Monteagudo, Bruno Contreras-Moreira in Scientific Reports (2023)

  3. Article

    Open Access

    GET_PANGENES: calling pangenes from plant genome alignments confirms presence-absence variation

    Crop pangenomes made from individual cultivar assemblies promise easy access to conserved genes, but genome content variability and inconsistent identifiers hamper their exploration. To address this, we define...

    Bruno Contreras-Moreira, Shradha Saraf, Guy Naamati, Ana M. Casas in Genome Biology (2023)

  4. No Access

    Protocol

    Pangenome Analysis of Plant Transcripts and Coding Sequences

    The pangenome of a species is the sum of the genomes of its individuals. As coding sequences often represent only a small fraction of each genome, analyzing the pangene set can be a cost-effective strategy for...

    Bruno Contreras-Moreira, Álvaro Rodríguez del Río in Plant Comparative Genomics (2022)

  5. Protocol

    Scripting Analyses of Genomes in Ensembl Plants

    Ensembl Plants (http://plants.ensembl.org) offers genome-scale information for plants, with four releases per year. As of release 47 (April 2020) it features 79 species ...

    Bruno Contreras-Moreira, Guy Naamati, Marc Rosello, James E. Allen in Plant Bioinformatics (2022)

  6. Article

    Open Access

    Gradual polyploid genome evolution revealed by pan-genomic analysis of Brachypodium hybridum and its diploid progenitors

    Our understanding of polyploid genome evolution is constrained because we cannot know the exact founders of a particular polyploid. To differentiate between founder effects and post polyploidization evolution,...

    Sean P. Gordon, Bruno Contreras-Moreira, Joshua J. Levy in Nature Communications (2020)

  7. Article

    Open Access

    Fine-tuning of the flowering time control in winter barley: the importance of HvOS2 and HvVRN2 in non-inductive conditions

    In winter barley plants, vernalization and photoperiod cues have to be integrated to promote flowering. Plant development and expression of different flowering promoter (HvVRN1, HvCO2, PPD-H1, HvFT1, HvFT3) and r...

    Arantxa Monteagudo, Ernesto Igartua, Bruno Contreras-Moreira in BMC Plant Biology (2019)

  8. No Access

    Article

    Resequencing theVrs1 gene in Spanish barley landraces revealed reversion of six-rowed to two-rowed spike

    Six-rowed spike 1 (Vrs1) is a gene of major importance for barley breeding and germplasm management as it is the main gene determining spike row-type (2-rowed vs. 6-rowed). This is a widely used DUS trait, and ha...

    Ana M. Casas, Bruno Contreras-Moreira, Carlos P. Cantalapiedra in Molecular Breeding (2018)

  9. Article

    Open Access

    Extensive gene content variation in the Brachypodium distachyon pan-genome correlates with population structure

    While prokaryotic pan-genomes have been shown to contain many more genes than any individual organism, the prevalence and functional significance of differentially present genes in eukaryotes remains poorly un...

    Sean P. Gordon, Bruno Contreras-Moreira, Daniel P. Woods in Nature Communications (2017)

  10. No Access

    Protocol

    FootprintDB: Analysis of Plant Cis-Regulatory Elements, Transcription Factors, and Binding Interfaces

    FootprintDB is a database and search engine that compiles regulatory sequences from open access libraries of curated DNA cis-elements and motifs, and their associated transcription factors (TFs). It systematic...

    Bruno Contreras-Moreira, Alvaro Sebastian in Plant Synthetic Promoters (2016)

  11. No Access

    Protocol

    RSAT::Plants: Motif Discovery in ChIP-Seq Peaks of Plant Genomes

    In this protocol, we explain how to run ab initio motif discovery in order to gather putative transcription factor binding motifs (TFBMs) from sets of genomic regions returned by ChIP-seq experiments. The protoco...

    Jaime A. Castro-Mondragon, Claire Rioualen in Plant Synthetic Promoters (2016)

  12. No Access

    Protocol

    RSAT::Plants: Motif Discovery Within Clusters of Upstream Sequences in Plant Genomes

    The plant-dedicated mirror of the Regulatory Sequence Analysis Tools (RSAT, http://plants.rsat.eu) offers specialized options for researchers dealing with plant tr...

    Bruno Contreras-Moreira, Jaime A. Castro-Mondragon in Plant Synthetic Promoters (2016)

  13. No Access

    Article

    BARLEYMAP: physical and genetic map** of nucleotide sequences and annotation of surrounding loci in barley

    The BARLEYMAP pipeline was designed to map both genomic sequences and transcripts against sequence-enriched genetic/physical frameworks, with plant breeders as the main target users. It reports the most proba...

    Carlos P. Cantalapiedra, Ridha Boudiar, Ana M. Casas, Ernesto Igartua in Molecular Breeding (2015)

  14. No Access

    Article

    Selection footprints in barley breeding lines detected by combining genoty**-by-sequencing with reference genome information

    This study is a retrospective analysis of an elite cross from the Spanish National Barley Breeding Program. This was the most successful cross produced in the breeding program in the past 20 years. The progeny...

    Ernesto Igartua, Elsayed Mansour, Carlos P. Cantalapiedra in Molecular Breeding (2015)

  15. No Access

    Protocol

    Robust Identification of Orthologues and Paralogues for Microbial Pan-Genomics Using GET_HOMOLOGUES: A Case Study of pIncA/C Plasmids

    GET_HOMOLOGUES is an open-source software package written in Perl and R to define robust core- and pan-genomes by computing consensus clusters of orthologous gene families from whole-genome sequences using the...

    Pablo Vinuesa, Bruno Contreras-Moreira in Bacterial Pangenomics (2015)

  16. No Access

    Article

    Loss of neuronal 3D chromatin organization causes transcriptional and behavioural deficits related to serotonergic dysfunction

    The interior of the neuronal cell nucleus is a highly organized three-dimensional (3D) structure where regions of the genome that are linearly millions of bases apart establish sub-structures with specialized ...

    Satomi Ito, Adriana Magalska, Manuel Alcaraz-Iborra in Nature Communications (2014)

  17. Article

    Open Access

    Integrating bioinformatic resources to predict transcription factors interacting with cis-sequences conserved in co-regulated genes

    Using motif detection programs it is fairly straightforward to identify conserved cis-sequences in promoters of co-regulated genes. In contrast, the identification of the transcription factors (TFs) interacting w...

    Christian Dubos, Zsolt Kelemen, Alvaro Sebastian, Lorenz Bülow, Gunnar Huep in BMC Genomics (2014)

  18. No Access

    Article

    Fine map** of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces

    In two Spanish barley landraces with outstanding resistance to scald, the Rrs1 Rh4 locus was fine ...

    Kerstin Hofmann, Cristina Silvar, Ana M. Casas in Theoretical and Applied Genetics (2013)

  19. Article

    Open Access

    Genetic recombination is associated with intrinsic disorder in plant proteomes

    Intrinsically disordered proteins, found in all living organisms, are essential for basic cellular functions and complement the function of ordered proteins. It has been shown that protein disorder is linked t...

    Inmaculada Yruela, Bruno Contreras-Moreira in BMC Genomics (2013)

  20. No Access

    Article

    OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors

    High salinity causes remarkable losses in rice productivity worldwide mainly because it inhibits growth and reduces grain yield. To cope with environmental changes, plants evolved several adaptive mechanisms, ...

    Tânia S. Serra, Duarte D. Figueiredo, André M. Cordeiro in Plant Molecular Biology (2013)

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