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Article
Open AccessMacroscale fluorescence imaging against autofluorescence under ambient light
Macroscale fluorescence imaging is increasingly used to observe biological samples. However, it may suffer from spectral interferences that originate from ambient light or autofluorescence of the sample or its...
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Article
Open AccessAuthor Correction: Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
The Peer Review File associated with this Article was updated shortly after publication to redact from the authors’ point-by-point response a description of unpublished work describing how Speed OPIOM may in f...
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Article
Open AccessResonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
We present speed out-of-phase imaging after optical modulation (OPIOM), which exploits reversible photoswitchable fluorophores as fluorescent labels and combines optimized periodic illumination with phase-sens...
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Article
Organocatalytic activity of [3]ferrocenophanes: a computational study
The organocatalytic activity of the recently synthesized diamino[3]ferrocenophanes and the related thioamino[3]ferrocenophane was investigated in the benzoin condensation computationally. The nucleophilicity o...
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Protocol
Fast and Efficient Cloning of Cis-Regulatory Sequences for High-Throughput Yeast One-Hybrid Analyses of Transcription Factors
Yeast one-hybrid (Y1H) assay has been proven to be a powerful technique to characterize in vivo the interaction between a given transcription factor (TF), or its DNA-binding domain (DBD), and target DNA sequen...
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Article
Vertical physico-chemical gradients with distinct microbial communities in the hypersaline and heliothermal Lake Ursu (Sovata, Romania)
The effect of vertical physico-chemical stratification on the planktonic microbial community composition of the deep, hypersaline and heliothermal Lake Ursu (Sovata, Romania) was examined in this study. On sit...
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Article
Open AccessIntegrating bioinformatic resources to predict transcription factors interacting with cis-sequences conserved in co-regulated genes
Using motif detection programs it is fairly straightforward to identify conserved cis-sequences in promoters of co-regulated genes. In contrast, the identification of the transcription factors (TFs) interacting w...
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Article
Penis covering and simultaneous urethral replacement by scrotal skin for severe penile and urethral necrosis
Penile strangulation with severe necrosis and concomitant significant tissue loss is a challenging problem for reconstructive surgery. Final reconstruction and tissue augmentation of the resected penis often r...
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Article
Renal tumor causing haematuria and sepsis
A 28 year old female patient developed hematuria in the 32th week of her pregnancy. She was given antibiotic treatment, since a urinary tract infection was suspected. After delivery symptoms of acute pyeloneph...
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Article
Transformation vector based on promoter and intron sequences of a replacement histone H3 gene. A tool for high, constitutive gene expression in plants
This study explored the possibility of using non-viral, plant-based gene sequences to create strong and constitutive expression vectors. Replacement histone H3 genes are highly and constitutively expressed in ...
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Article
Expression of a novel-type small proline-rich protein gene of alfalfa is induced by 2,4-dichlorophenoxiacetic acid in dedifferentiated callus cells
Differential screening of a cDNA library of 2,4-dichlorophenoxiacetic acid (2,4-D)-treated alfalfa (Medicago sativa) callus tissues resulted in the isolation of a 571 bp cDNA clone (MsPRP5) encoding for a prol...
