93 Result(s)
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Life Sciences
Microbiology
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Article
A functional chimeric membrane subunit of an ion-translocating ATPase
A chimeric transport protein was made by expression of a fusion of thearsB genes fromEscherichia coli plasmid R773 andStaphylococcus aureus plasmid pI258. The two genes were fused to encode a functional protein w...
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Article
Characterization and gene cloning of a novel β-mannanase from alkaliphilic Bacillus sp. N16-5
An alkaline β-mannanase was purified to homogeneity from a culture broth of alkaliphilic Bacillus sp. N16-5. The enzyme had optimum activity at pH 9.5 and 70°C. It was composed of a single polypeptide chain with ...
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Remediation of pentachlorophenol-contaminated soil by composting with immobilized Phanerochaete chrysosporium
To reduce and eliminate the hazards of pentachlorophenol (PCP) to the soil, the method of inoculating free and immobilized white rot fungi, Phanerochaete chrysosporium to PCP-polluted soils was investigated. Thre...
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Optimization of medium composition for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5 using response surface methodology
In this work, a 22 factorial design was employed combining with response surface methodology (RSM) to optimize the medium compositions for the production of alkaline β-mannanase by alkaliphilic Bacillus sp. N16-5...
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Biosorption of cadmium by a Cd2+-hyperresistant Bacillus cereus strain HQ-1 newly isolated from a lead and zinc mine
A Cd2+-hyperresistant bacterial strain HQ-1 was isolated from a lead–zinc mine. The strain was characterized and identified as Bacillus cereus based on morphology, physiological tests and 16S rRNA gene analysis. ...
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Tumor cellular proteasome inhibition and growth suppression by 8-hydroxyquinoline and clioquinol requires their capabilities to bind copper and transport copper into cells
We have previously reported that when mixed with copper, 8-hydroxyquinoline (8-OHQ) and its analog clioquinol (CQ) inhibited the proteasomal activity and proliferation in cultured human cancer cells. CQ treatm...
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Cloning, expression, purification, and activity assay of proteins related to D-lactic acid formation in Lactobacillus rhamnosus
Two proteins that might be responsible for D-lactic acid (D-LA) formation were screened from the genome database of Lactobacillus rhamnosus GG. The coding genes of the two proteins in L. rhamnosus CASL, ldhD1 and...
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PCR methods for the rapid detection and identification of four pathogenic Legionella spp. and two Legionella pneumophila subspecies based on the gene amplification of gyrB
A total of 25 gyrB gene sequences from 20 Legionella pneumophila subsp. pneumophila strains and five L. pneumophila subsp. fraseri strains were obtained and analyzed, and a multiplex PCR for the simultaneous dete...
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Article
Arbuscular mycorrhiza regulate inter-specific competition between a poisonous plant, Ligularia virgaurea, and a co-existing grazing grass, Elymus nutans, in Tibetan Plateau Alpine meadow ecosystem
In order to investigate the function of arbuscular mycorrhizae in Tibetan Plateau Alpine meadow ecosystems, experiments were carried out to evaluate the influence of arbuscular mycorrhizal (AM) fungi on inter-...
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Article
Site-Directed Mutagenesis Studies on the l-Arginine-Binding Sites of Feedback Inhibition in N-Acetyl-l-glutamate Kinase (NAGK) from Corynebacterium glutamicum
Arginine biosynthesis in Corynebacterium glutamicum proceeds via a pathway that is controlled by arginine through feedback inhibition of NAGK, the enzyme that converts N-acetyl-l-glutamate (NAG) to N-acety-l-glut...
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Article
Heterologous and homologous expression of the arginine biosynthetic argC~H cluster from Corynebacterium crenatum for improvement of l-arginine production
The genes involved in l-arginine biosynthesis in Corynebacterium crenatum are organized as the argCJBDFRGH cluster like in Corynebacterium glutamicum. However, the argC~H cluster of the C. crenatum SYPA 5-5, whic...
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Article
Strain improvement for enhanced production of S-adenosyl-L-methionine in Saccharomyces cerevisiae based on ethionine-resistance and SAM synthetase activity
S-adenosyl-L-methionine (SAM) is an essential metabolite in all living cells, and which plays an important role in cellular functions such as methylation, sulfuration, and polyamine synthesis. The current stud...
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1,10-Phenanthroline promotes copper complexes into tumor cells and induces apoptosis by inhibiting the proteasome activity
Indole-3-acetic acid and indole-3-propionic acid, two potent natural plant growth hormones, have attracted attention as promising prodrugs in cancer therapy. Copper is known to be a cofactor essential for tumo...
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The Effect of a LYSE Exporter Overexpression on l-Arginine Production in Corynebacterium crenatum
Corynebacterium crenatum SYPA is an aerobic and industrial l-arginine producer. A lysE deletion mutant was constructed searching a natural function of this in C. crenatum SYPA. Tracking determina...
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RETRACTED ARTICLE: Human leptin triggers proliferation of A549 cells via blocking endoplasmic reticulum stress-related apoptosis
Lung cancer is a disease characterized by uncontrolled cell growth in tissues of the lung. Leptin is a pleiotropic hormone with antiapoptotic and proliferative roles involved in...
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Signatures of positive selection in LY96 gene in vertebrates
As a secreted glycoprotein that binds to the extracellular domain of Toll-like receptor 4 (TLR4), Lymphocyte Antigen 96 (LY96), also called myeloid differentiation 2 (MD2), is required for the activation of TLR4 ...
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Article
Evidence of positive selection at codon sites localized in the C-terminal peptide of ORC6
Origin recognition complex 6 (Orc6) plays a central role in the initiation of DNA replication in all eukaryotic systems. The exact contribution of Orc6 to replication initiation has yet to be elucidated. Here,...
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Functional analysis of a subtilisin-like serine protease gene from biocontrol fungus Trichoderma harzianum
The subtilisin-like serine protease gene ThSS45 has been cloned from Trichoderma harzianum ACCC30371. Its coding region is 1302 bp in length, encoding 433 amino acids, with a predicted protein molecular weight of...
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Retraction Note to: “Human leptin triggers proliferation of a549 cells via blocking endoplasmic reticulum stress-related apoptosis”
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Article
Recombinant expression of glpK and glpD genes improves the accumulation of shikimic acid in E. coli grown on glycerol
Shikimic acid (SA) is an industrially important chiral compound used in diverse commercial applications, and the insufficient supply by isolation from plants and expensive chemical synthesis of SA has increase...
