-
Article
Recycling of spent liquor for treating corn cobs to create digestible cellulose and enrich the xylooligosaccharide concentration
To reduce the cost of pretreatment, an advanced formic acid pretreatment directly using spent liquor was developed for corn cob valorisation in this work. The spent liquor pretreatment showed a satisfactory li...
-
Article
Enhancing enzymatic hydrolysis of Chinese fir sawdust by using the synergistic effect of dilute sulfuric acid and sodium chlorite pretreatment
The biological valorization of cork depends mainly on the weakening of cell wall recalcitrance. As a typical softwood, Chinese fir is more resistant to enzyme and microbial invasion than most biomass. This study ...
-
Article
Open AccessEfficient biosynthesis of pinosylvin from lignin-derived cinnamic acid by metabolic engineering of Escherichia coli
The conversion of lignin-derived aromatic monomers into valuable chemicals has promising potential to improve the economic competitiveness of biomass biorefineries. Pinosylvin is an attractive pharmaceutical w...
-
Article
Open AccessValorization of cheese whey to lactobionic acid by a novel strain Pseudomonas fragi and identification of enzyme involved in lactose oxidation
Efficient upgrading of inferior agro-industrial resources and production of bio-based chemicals through a simple and environmentally friendly biotechnological approach is interesting Lactobionic acid is a vers...
-
Article
Open AccessEfficient lactic acid production from dilute acid-pretreated lignocellulosic biomass by a synthetic consortium of engineered Pseudomonas putida and Bacillus coagulans
Lignocellulosic biomass is an attractive and sustainable alternative to petroleum-based feedstock for the production of a range of biochemicals, and pretreatment is generally regarded as indispensable for its ...
-
Article
Mild and efficient two-step pretreatment of lignocellulose using formic acid solvent followed by alkaline salt
An efficient fractionation of three main components of corn cob and a good delignification effect were reported with formic acid (88%) pretreatment at 80 °C for 3 h. The pretreated substrate demonstrated a poo...
-
Article
Open AccessEfficient biosynthesis of cinnamyl alcohol by engineered Escherichia coli overexpressing carboxylic acid reductase in a biphasic system
Cinnamyl alcohol is not only a kind of flavoring agent and fragrance, but also a versatile chemical applied in the production of various compounds. At present, the preparation of cinnamyl alcohol depends on pl...
-
Article
A versatile Pseudomonas putida KT2440 with new ability: selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid
Currently, biotransformation of 5-hydroxymethylfurfural (HMF) into a series of high-value bio-based platform chemicals is massively studied. In this study, selective biooxidation of HMF to 5-hydroxymethyl-2-fu...
-
Article
Development of two-step pretreatment of Chinese fir sawdust using dilute sulfuric acid followed by sodium chlorite for bioethanol production
Chinese fir sawdust (CFS) is an abundant potential feedstock for bio-ethanol production. However, as a sawdust from this softwood, its high recalcitrance to enzymatic hydrolysis limits its successful applicati...
-
Article
Open AccessSimultaneous consumption of cellobiose and xylose by Bacillus coagulans to circumvent glucose repression and identification of its cellobiose-assimilating operons
The use of inedible lignocellulosic biomasses for biomanufacturing provides important environmental and economic benefits for society. Efficient co-utilization of lignocellulosic biomass-derived sugars, primar...
-
Article
Enhanced Inulin Saccharification by Self-Produced Inulinase from a Newly Isolated Penicillium sp. and its Application in d-Lactic Acid Production
In order to find an alternative for commercial inulinase, a strain XL01 identified as Penicillium sp. was screened for inulinase production. The broth after cultivated was centrifuged, filtered, and used as crude...
-
Article
Enhanced biosynthesis of chiral phenyllactic acid from l-phenylalanine through a new whole-cell biocatalyst
Phenyllactic acid (PLA) is a high-value compound, which was usually produced by lactic acid bacteria (LAB) as biocatalysts and glucose or phenylpyruvic acid (PPA) as starting materials for PLA synthesis in pre...
-
Article
Efficient in situ separation and production of l-lactic acid by Bacillus coagulans using weak basic anion-exchange resin
To get rid of the dependence on lactic acid neutralizer, a simple and economical approach for efficient in situ separation and production of l-lactic acid was established by Bacillus coagulans using weak basic an...
-
Article
Metabolic Engineering of Escherichia coli K12 for Homofermentative Production of l-Lactate from Xylose
The efficient utilization of xylose is regarded as a technical barrier to the commercial production of bulk chemicals from biomass. Due to the desirable mechanical properties of polylactic acid (PLA) depending...
-
Article
Genomic analysis of a xylose operon and characterization of novel xylose isomerase and xylulokinase from Bacillus coagulans NL01
To investigate the xylose operon and properties of xylose isomerase and xylulokinase in Bacillus coagulans that can effectively ferment xylose to lactic acid.
-
Article
Open AccessCharacterization of an L-arabinose isomerase from Bacillus coagulans NL01 and its application for D-tagatose production
L-arabinose isomerase (AI) is a crucial catalyst for the biotransformation of D-galactose to D-tagatose. In previous reports, AIs from thermophilic bacterial strains had been wildly researched, but the brownin...
-
Article
Molecular Characterization of a Recombinant Zea mays Phenylalanine Ammonia-Lyase (ZmPAL2) and Its Application in trans-Cinnamic Acid Production from l-Phenylalanine
Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes with its crucial role in secondary phenylpropanoid metabolism of plants. Recently, its demand has been increased for aromatic ch...
-
Article
Enhanced l-Lactic Acid Production from Biomass-Derived Xylose by a Mutant Bacillus coagulans
Xylose effective utilization is crucial for production of bulk chemicals from low-cost lignocellulosic substrates. In this study, an efficient l-lactate production process from xylose by a mutant Bacillus coagula...
-
Article
Open AccessHighly stereoselective biosynthesis of (R)-α-hydroxy carboxylic acids through rationally re-designed mutation of d-lactate dehydrogenase
An NAD-dependent d-lactate dehydrogenase (d-nLDH) of Lactobacillus bulgaricus ATCC 11842 was rationally re-designed for asymmetric reduction of a homologous series of α-keto carboxylic acids such as phenylpyruvic...
-
Article
Efficient Non-sterilized Fermentation of Biomass-Derived Xylose to Lactic Acid by a Thermotolerant Bacillus coagulans NL01
Xylose is the major pentose and the second most abundant sugar in lignocellulosic feedstock. Its efficient utilization is regarded as a technical barrier to the commercial production of bulk chemicals from lig...
