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Article
Enhanced Production of Sisomicin in Micromonospora inyoensis by Protoplast Mutagenesis and Fermentation Optimization
Sisomicin is a broad-spectrum aminoglycoside antibiotic and is the precursor of netilmicin and plazomicin. However, the fermentation level of sisomicin is still low compared with other antibiotics, which restr...
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Article
Enhancing the biotransformation efficiency of human CYP17A1 in Pichia pastoris by co-expressing CPR and glucose-6-phosphate dehydrogenase simultaneously
Steroids are a widely used class of drugs, and their hydroxylation modification has important pharmacological significance. Cytochrome P450 is the core enzyme for hydroxylation modification of steroid molecule...
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Article
Molecular dynamics-based study of the modification mechanism of asphalt by graphene oxide
Graphene oxide(GO) has been widely used in asphalt modification due to its excellent properties. To reveal the interaction effect between GO and asphalt materials, the microscopic behavior and molecular struct...
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Article
Open AccessResearch on the synergistic modification effect and the interface mechanism of GO/SBS compound-modified asphalt based on experiments and molecular simulations
Although there have been reports showing the modification effect of carbon nanomaterials on asphalt, there are few studies on whether carbon nanomaterials and polymers can have synergistic modification effects...
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Article
Characterization and implications of prokaryotic ribosome-binding sites across species
The ribosome-binding site (RBS) in the 5′ untranslated region is recognized by 16S rRNA to start translation and is an essential element of the gene expression system. RBSs have been widely applied in regulati...
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Article
Extended State Observer Based Adaptive Backstep** Nonsingular Fast Terminal Sliding-mode Control for Robotic Manipulators with Uncertainties
This paper proposes a novel robust finite-time tracking control scheme for robotic manipulators with uncertainties and external disturbances. In order to reduce the chattering phenomenon and enhance the robust...
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Article
Backstep** Sliding Mode Control Based on Extended State Observer for Robotic Manipulators with LuGre Friction
In this paper, we propose a backstep** integral nonsingular fast terminal sliding mode control approach based on an extended state observer to the trajectory tracking control of robotic manipulators with LuG...
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Article
Improving glutathione production by engineered Pichia pastoris: strain construction and optimal precursor feeding
Glutathione (GSH) is a metabolite that plays an important role in the fields of pharmacy, food, and cosmetics. Thus, it is necessary to increase its production to meet the demands. In this study, ScGSH1, ScGSH2, ...
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Article
An Adaptive Fault-tolerant Control Method for Robot Manipulators
This paper presents a new method for adaptive continuous nonsingular fast terminal sliding mode control (ACNFTSMC) based on a novel structure-improved extended state observer (SIESO) for fault-tolerant control...
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Article
Similarities and differences of oligo/poly-saccharides’ impact on human fecal microbiota identified by in vitro fermentation
The dietary supplementation of prebiotics is considered a promising strategy for the modulation of gut microbiota. Due to the wide variety of animal models and tremendous inter-individual variability from huma...
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Article
Enhanced production of L-arginine by improving carbamoyl phosphate supply in metabolically engineered Corynebacterium crenatum
Carbamoyl phosphate is an important precursor for L-arginine and pyrimidines biosynthesis. In view of this importance, the cell factory should enhance carbamoyl phosphate synthesis to improve related compound ...
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Article
Open AccessHigh-yield production of l-serine through a novel identified exporter combined with synthetic pathway in Corynebacterium glutamicum
l-Serine has wide and increasing applications in industries with fast-growing market demand. Although strategies for achieving and improving l-serine production in Corynebacterium glutamicum (C. glutamicum) have ...
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Article
Open AccessThe effects of add-on self-care education on quality of life and fatigue in gastrointestinal cancer patients undergoing chemotherapy
Gastrointestinal cancer is one of the most common malignancies and imposes heavy burdens on both individual health and social economy. We sought to survey the effect of a self-care education program on quality...
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Article
Enhancement of l-arginine production by increasing ammonium uptake in an AmtR-deficient Corynebacterium crenatum mutant
l-Arginine is an important amino acid with extensive application in the food and pharmaceutical industries. The efficiency of nitrogen uptake and assimilation by organisms is extremely important for l-arginine pr...
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Article
Correction to: High-yield production of l-serine from glycerol by engineered Escherichia coli
Unfortunately, the order of the figures 1-4 has been positioned wrongly in the print published article
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Article
Identification of steroid C27 monooxygenase isoenzymes involved in sterol catabolism and stepwise pathway engineering of Mycobacterium neoaurum for improved androst-1,4-diene-3,17-dione production
Cholesterol oxidase, steroid C27 monooxygenase and 3-ketosteroid-Δ1-dehydrogenase are key enzymes involved in microbial catabolism of sterols. Here, three isoenzymes of steroid C27 monooxygenase were firstly char...
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Article
Enhancing Expression of 3-Ketosteroid-9α-Hydroxylase Oxygenase, an Enzyme with Broad Substrate Range and High Hydroxylation Ability, in Mycobacterium sp. LY-1
3-Ketosteroid-9α-hydroxylase (KSH) consists of two protein systems, KshA and KshB, and is a key enzyme in microbial degradation pathway of natural sterols. 9α-Hydroxy-4-androstene-3,17-dione (9α-OH-AD) is a va...
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Article
High-yield production of l-serine from glycerol by engineered Escherichia coli
l-Serine is widely used in pharmaceutical, food and cosmetic industries, and the direct fermentation to produce l-serine from cheap carbon sources such as glycerol is greatly desired. The production of l-serine b...
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Article
A Novel 2-Keto-d-Gluconic Acid High-Producing Strain Arthrobacter globiformis JUIM02
2-Keto-d-gluconic acid (2KGA) is mainly used for industrial production of erythorbic acid, a food antioxidant. In this study, a 2KGA producing strain JUIM02 was firstly identified as Arthrobacter globiformis by m...
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Article
pDHS-ELM: computational predictor for plant DNase I hypersensitive sites based on extreme learning machines
DNase I hypersensitive sites (DHSs) are hallmarks of chromatin zones containing transcriptional regulatory elements, making them critical in understanding the regulatory mechanisms of gene expression. Although...
