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Article
Open AccessAn oxylipin signal confers protection against antifungal echinocandins in pathogenic aspergilli
Aspergillus fumigatus is the leading causative agent of life-threatening invasive aspergillosis in immunocompromised individuals. One antifungal class used to treat Aspergillus infections is the fungistatic echin...
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Article
Correlative metabologenomics of 110 fungi reveals metabolite–gene cluster pairs
Natural products research increasingly applies -omics technologies to guide molecular discovery. While the combined analysis of genomic and metabolomic datasets has proved valuable for identifying natural prod...
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Article
Open AccessCopper starvation induces antimicrobial isocyanide integrated into two distinct biosynthetic pathways in fungi
The genomes of many filamentous fungi, such as Aspergillus spp., include diverse biosynthetic gene clusters of unknown function. We previously showed that low copper levels upregulate a gene cluster that includes...
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Article
Open AccessFungal oxylipins direct programmed developmental switches in filamentous fungi
Filamentous fungi differentiate along complex developmental programs directed by abiotic and biotic signals. Currently, intrinsic signals that govern fungal development remain largely unknown. Here we show tha...
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Article
Open AccessLipo-chitooligosaccharides as regulatory signals of fungal growth and development
Lipo-chitooligosaccharides (LCOs) are signaling molecules produced by rhizobial bacteria that trigger the nodulation process in legumes, and by some fungi that also establish symbiotic relationships with plant...
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Article
Open AccessGenome sequencing of evolved aspergilli populations reveals robust genomes, transversions in A. flavus, and sexual aberrancy in non-homologous end-joining mutants
Aspergillus spp. comprises a very diverse group of lower eukaryotes with a high relevance for industrial applications and clinical implications. These multinucleate species are often cultured for many generations...
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Article
A scalable platform to identify fungal secondary metabolites and their gene clusters
Coupling the use of artificial chromosomes with metabolomics enables the high-throughput linkage of fungal natural products with their biosynthetic gene clusters. This method was used here to identify a novel...
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Article
Plant-like biosynthesis of isoquinoline alkaloids in Aspergillus fumigatus
Analysis of orphan nonribosomal peptide synthetase–like gene clusters from Aspergillus fumigatus identified a gene cluster responsible for the biosynthesis of fumisoquin isoquinoline alkaloids by amino acid conde...
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Article
Open AccessFungal artificial chromosomes for mining of the fungal secondary metabolome
With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30–80 kb in size, breakthrough techniques are needed to characterize this SM wealth.
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Article
Open AccessIllumina identification of RsrA, a conserved C2H2 transcription factor coordinating the NapA mediated oxidative stress signaling pathway in Aspergillus
Chemical mutagenesis screens are useful to identify mutants involved in biological processes of interest. Identifying the mutation from such screens, however, often fails when using methodologies involving tra...
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Article
Correction: Corrigendum: Chromatin-level regulation of biosynthetic gene clusters
Nat. Chem. Biol. 5, 462–464 (2009); published online 17 May 2009; corrected after print 18 August 2009 In the version of this article initially published, the fourth author's last name is misspelled. The autho...
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Chromatin-level regulation of biosynthetic gene clusters
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Article
Kalilo plasmids are a family of four distinct members with individual global distributions across species
Kalilo is a linear 9-kb plasmid, isolated originally from Hawaiian strains of the heterothallic fungus Neurospora intermedia. Its properties include terminal inverted repeats, two ORFs coding for a presumptive D...
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Article
Transfer of Neurospora kalilo plasmids among species and strains by introgression
There are four different variants of the kalilo “family” of linear mitochondrial plasmids. This family is found in several heterothallic species and one pseudohomothallic species of Neurospora, as well as in one...
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Article
Studies on protoplast fusion betweenLentinula edodes andGanoderma lucidum
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Article
Conformation of group “a” epitope in hepatitis B surface antigen
To elucidate structure of group “a” epitope, mouse antibodies that express idiotype monoclonal antibody and anti-idiotype monoclonal antibody against the group-specific “a” determinant were purified by hydroxy...
