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Article
Antioxidant activity of cyanidins isolated from Ogapy (Acanthopanax divaricatus var. albeofructus) fruits in U937 macrophages
Reactive oxygen species (ROS) are continuously produced in aerobic organisms. Overproduction of ROS is known to play a crucial role in the pathogenesis of many cardiovascular diseases, including atherosclerosi...
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Article
Gene network analysis on the effect of Viscum album var. coloratum in T cells stimulated with anti-CD3/CD28 antibodies
A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. var. coloratum agglutinin, VCA), which is known for its anticancer activity, was isolated from Korean mistletoe. This study reports a mic...
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Article
Mistletoe lectin transport by M-cells in follicle-associated epithelium (FAE) and IL-12 secretion in dendritic cells situated below FAE In Vitro
A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. var. coloratum agglutinin, VCA), which is known for its anti-tumor activity, was isolated from Korean mistletoe. Mistletoe preparations h...
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Article
Mistletoe lectin modulates intestinal epithelial cell-derived cytokines and B cell IgA secretion
A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. var. coloratum agglutinin, VCA), which is known for its anti-cancer activity, was isolated from Korean mistletoe. In this study, IEC-6 ra...
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Article
Transport of mistletoe lectin by M cells in human intestinal follicle-associated epithelium (FAE) In vitro
Purified mistletoe lectins are known to have cytotoxic and stimulating activities in the immune system. Mistletoe extract has been given subcutaneously because of its unstablity and poor absorption in the Gast...
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Article
Effects of Korean Mistletoe Lectin(Viscum album coloratum) on Proliferation and Cytokine Expression in Human Peripheral Blood Mononuclear Cells and T-Lymphocytes
The anti-cancer activity of mistletoe has been ascribed to a combination of cytotoxic and immunological effects. We previously showed that Korean mistletoe lectin(Viscum album L.var. coloratum agglutinin, VCA) ca...
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Article
Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2)in vitro
Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant, anticancer, bactericidal, and anti-inflammatory. We carried out anti-herpetic assa...
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Article
Production of Cytokine and NO by RAW 264.7 Macrophages and PBMC In Vitro Incubation with Flavonoids
Flavonoids, a group of low molecular weight phenylbenzopyrones, have various pharmacological properties including antioxidant activity, anticancer, and immunomodulatory effects. In the present study, lipopolys...
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Article
Mistletoe lectin induces apoptosis and telomerase inhibition in human A253 cancer cells through dephosphorylation of akt
Mistletoe lectin has been reported to induce apoptosis in different cancer cell linesin vitro and to show antitumor activity against a variety of tumors in animal models. We previously demonstrated the Korean mis...
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Article
Preparation of alginate/chitosan microcapsules and enteric coated granules of mistletoe lectin
The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cyto-toxic and immune-system-stimulati...
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Article
Korean mistletoe lectin-induced apoptosis in hepatocarcinoma cells is associated with inhibition of telomerasevia mitochondrial controlled pathway independent of p53
The extract of European mistletoe(Viscum album, L) has been used in adjuvant chemotherapy of cancer and mistletoe lectins are considered to be major active components. The present work was performed to investigat...
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Article
Comparative study of korean (Viscum album var.coloratum) and european mistletoes (Viscum album)
A lectin (agglutinin, VCA) from Korean mistletoe (Viscum album L.coloratum) was isolated by affinity chromatograpy on a asialofetuin-Sepharose 4B. The molecular weights of A-and B-chains of VCA were differenf fro...
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Article
Isolation and characterization of β-galactoside specific lectin from Korean mistletoe (Viscum album var.coloratum) with lactose-BSA-Sepharose 4B and changes of lectin conformation
Lectins and its A- and B-chains from Korean mistletoe (Viscum album var.coloratum) were isolated by affinity chromatography on the Sepharose 4B modified by lactose-BSA conjugate synthesized by reductive amination...
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Article
Isolation and characterization of lectins from stem and leaves of Korean mistletoe (Viscum album var.coloratum) by affinity chromatography
We attempted to isolate and characterize the lectins from stem and leaves of Korean mistletoe (Viscum album var.coloratum) by affinity chromatography. Lectin I was isolated only from stem. Lectin II was not isola...
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Article
Enhancement of water-solubilities of protein-bound polysaccharides contained in the basidiocarps ofGanoderma lucidum by hydrolyzing with chymotrypsin
Optimum conditions for hydrolysis were investigated to enhance water-solubilities of protein-bound polysaccharides in the basidiocarps ofGanoderma lucidum by treating chymotrypsin. We also attempted withGanoderma...
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Article
Conversion of water-insoluble components of the basidiocarps ofGanoderma lucidum to water-soluble components by hydrolyzing with chitinase
We investigated the optimum conditions for conversion of water-insoluble components of basidiocarps ofGanoderma lucidum to water-soluble components by hydrolyzing with chitinase. We also tried it withGanoderma lu...
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Article
Conformation of group “a” epitope in hepatitis B surface antigen
To elucidate structure of group “a” epitope, mouse antibodies that express idiotype monoclonal antibody and anti-idiotype monoclonal antibody against the group-specific “a” determinant were purified by hydroxy...
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Article
Production of non-overlapped monoclonal antibodies against hepatitis B surface antigen and their use in two-site enzyme linked immunosorbant assay (ELISA)
Eleven stable hybridoma cell lines secreting antibodies against HBsAg were established. Six out of 11 clones reacted with the common “a” determinant of HBsAg. The six monoclonal antibodies were conjugated to h...
