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Article
Correlative metabologenomics of 110 fungi reveals metabolite–gene cluster pairs
Natural products research increasingly applies -omics technologies to guide molecular discovery. While the combined analysis of genomic and metabolomic datasets has proved valuable for identifying natural prod...
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Article
Open AccessRapid counting and spectral sorting of live coral larvae using large-particle flow cytometry
Research with coral embryos and larvae often requires laborious manual counting and sorting of individual specimens, usually via microscopy. Because many coral species spawn only once per year during a narrow ...
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Article
Open AccessImmobilisation of living coral embryos and larvae
Embedding and immobilisation of living cells and microorganisms is used in a variety of research and commercial applications. Here we report the successful extended immobilisation of coral larvae in a low-gell...
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Article
New interventions are needed to save coral reefs
We anticipate that conventional management approaches will be insufficient to protect coral reefs, even if global warming is limited to 1.5 °C. Emerging technologies are needed to stem the decline of these nat...
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Article
Open AccessComplete genome of Staphylococcus aureus Tager 104 provides evidence of its relation to modern systemic hospital-acquired strains
Staphylococcus aureus (S. aureus) infections range in severity due to expression of certain virulence factors encoded on mobile genetic elements (MGE). As such, characterization of the...
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Article
Rapid screening for protein solubility and expression
The Expresso® Solubility and Expression Screening System uses a simple ligase-free cloning strategy and a single PCR product to construct and test seven distinct protein fusion partners in parallel over the cours...
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Article
Open AccessFungal artificial chromosomes for mining of the fungal secondary metabolome
With thousands of fungal genomes being sequenced, each genome containing up to 70 secondary metabolite (SM) clusters 30–80 kb in size, breakthrough techniques are needed to characterize this SM wealth.
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Reference Work Entry In depth
Use of Viral Metagenomes from Yellowstone Hot Springs to Study Phylogenetic Relationships and Evolution
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Reference Work Entry In depth
Functional Viral Metagenomics and the Development of New Enzymes for DNA and RNA Amplification and Sequencing
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Living Reference Work Entry In depth
Functional Viral Metagenomics and the Development of New Enzymes for DNA and RNA Amplification and Sequencing
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Living Reference Work Entry In depth
Use of Viral Metagenomes from Yellowstone Hot Springs to Study Phylogenetic Relationships and Evolution
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Article
Endotoxin-free protein production—ClearColi™ technology
A new Escherichia coli strain with a genetically modified lipopolysaccharide (LPS) molecule has been created that enables protein expression without the endotoxin. Proteins expressed and purified from ClearColi™ ...
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Article
Long-span, mate-pair scaffolding and other methods for faster next-generation sequencing library creation
The NxSeq™ 40 kb Mate-Pair Cloning Kit facilitates the creation of scaffolds for de novo genome assembly. Supporting either Illumina or 454 sequencing, the kit produces long-span, mate-pair sequences with greater...
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Article
Erratum: Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes
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Article
Functional Annotation of Fibrobacter succinogenes S85 Carbohydrate Active Enzymes
Fibrobacter succinogenes is a cellulolytic bacterium that degrades plant cell wall biomass in ruminant animals and is among the most rapidly fibrolytic of all mesophilic bacteria. The complete genome sequence of
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Article
Open AccessHemicellulases and auxiliary enzymes for improved conversion of lignocellulosic biomass to monosaccharides
High enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the enzyme cocktail for specific types of pretre...
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Article
Mining Dictyoglomus turgidum for Enzymatically Active Carbohydrases
The genome of Dictyoglomus turgidum was sequenced and analyzed for carbohydrases. The broad range of carbohydrate substrate utilization is reflected in the high number of glycosyl hydrolases, 54, and the high per...
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Protocol
Isolation Independent Methods of Characterizing Phage Communities 2: Characterizing a Metagenome
Current appreciation of the vast expanse of prokaryotic diversity has largely come through molecular phylogenetic exploration of sequence diversity within the universally conserved gene for small subunit ribos...
