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Article
Open AccessAutomatic beam optimization method for scanning electron microscopy based on electron beam Kernel estimation
Scanning Electron Microscopy (SEM) leverages electron wavelengths for nanoscale imaging, necessitating precise parameter adjustments like focus, stigmator, and aperture alignment. However, traditional methods ...
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Article
Constitutive activation mechanism of a class C GPCR
Class C G-protein-coupled receptors (GPCRs) are activated through binding of agonists to the large extracellular domain (ECD) followed by rearrangement of the transmembrane domains (TMDs). GPR156, a class C or...
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Article
Open AccessPinostilbene inhibits full-length and splice variant of androgen receptor in prostate cancer
Prostate cancer is the most prevalent cancer in men worldwide and is promoted by the sex hormone androgen. Expression of androgen from the testis can be significantly reduced through castration. However, as mo...
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Article
Open AccessA stepwise activation model for the insulin receptor
The binding of insulin to the insulin receptor (IR) triggers a cascade of receptor conformational changes and autophosphorylation, leading to the activation of metabolic and mitogenic pathways. Recent advances...
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Article
Open AccessRegulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1
Aminoacyl-tRNA synthetases (ARSs) have evolved to acquire various additional domains. These domains allow ARSs to communicate with other cellular proteins in order to promote non-translational functions. Verte...
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Article
Open AccessFunctional selectivity of insulin receptor revealed by aptamer-trapped receptor structures
Activation of insulin receptor (IR) initiates a cascade of conformational changes and autophosphorylation events. Herein, we determined three structures of IR trapped by aptamers using cryo-electron microscopy...
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Article
Open AccessStructural basis for receptor selectivity and inverse agonism in S1P5 receptors
The bioactive lysophospholipid sphingosine-1-phosphate (S1P) acts via five different subtypes of S1P receptors (S1PRs) - S1P1-5. S1P5 is predominantly expressed in nervous and immune systems, regulating the egres...
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Article
Open AccessMicrosphere-assisted, nanospot, non-destructive metrology for semiconductor devices
As smaller structures are being increasingly adopted in the semiconductor industry, the performance of memory and logic devices is being continuously improved with innovative 3D integration schemes as well as ...
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Article
Open AccessStructure of the class C orphan GPCR GPR158 in complex with RGS7-Gβ5
GPR158, a class C orphan GPCR, functions in cognition, stress-induced mood control, and synaptic development. Among class C GPCRs, GPR158 is unique as it lacks a Venus flytrap-fold ligand-binding domain and te...
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Article
Open AccessPolyimide mesh-based sample holder with irregular crystal mounting holes for fixed-target serial crystallography
The serial crystallography (SX) technique enables the determination of the room-temperature structure of a macromolecule while causing minimal radiation damage, as well as the visualization of the molecular dy...
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Article
Open AccessNylon mesh-based sample holder for fixed-target serial femtosecond crystallography
Fixed-target serial femtosecond crystallography (FT-SFX) was an important advance in crystallography by dramatically reducing sample consumption, while maintaining the benefits of SFX for obtaining crystal str...
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Article
Junction resolving enzymes use multivalency to keep the Holliday junction dynamic
Holliday junction (HJ) resolution by resolving enzymes is essential for chromosome segregation and recombination-mediated DNA repair. HJs undergo two types of structural dynamics that determine the outcome of ...
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Article
Open AccessPolyacrylamide injection matrix for serial femtosecond crystallography
Serial femtosecond crystallography (SFX) provides opportunities to observe the dynamics of macromolecules without causing radiation damage at room temperature. Although SFX provides a biologically more reliabl...
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Article
Eukaryotic Rad50 functions as a rod-shaped dimer
Structural, biochemical and in vivo analyses reveal that Rad50 zinc-hook and coiled-coil domains form a novel dimerization interface essential for Mre11-complex function in DNA damage response and repair.
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Article
Crystal structure of human DGCR8 core
A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for ...
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Article
Structural basis for inhibition of the replication licensing factor Cdt1 by geminin
To maintain chromosome stability in eukaryotic cells, replication origins must be licensed by loading mini-chromosome maintenance (MCM2–7) complexes once and only once per cell cycle1,2,3,4,5,6,7,8,9. This licens...
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Article
Structure-based identification of a novel NTPase from Methanococcus jannaschii
Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemic...
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Article
Structure and mechanism of glutamate racemase from Aquifex pyrophilus
Glutamate racemase (MurI) is responsible for the synthesis of D-glutamate, an essential building block of the peptidoglycan layer in bacterial cell walls. The crystal structure of glutamate racemase from Aquifex ...
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Article
The crystal structure of flap endonuclease-1 from Methanococcus jannaschii
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Å reso...
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Article
Structural similarity between the pocket region of retinoblastoma tumour suppressor and the cyclin-box
The pocket region of retinoblastoma tumour suppressor (Rb) is essential for tumour suppressing activity. The Rb pocket is primarily composed of two domains, A and B. We have determined the X-ray crystal struct...
