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Protocol
PAM-Less CRISPR-SpRY Genome Editing in Plants
Engineered SpCas9 variant, SpRY, has been demonstrated to facilitate protospacer adjacent motif (PAM) unrestricted targeting of genomic DNA in various biological systems. Here we describe fast, efficient, and ...
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Protocol
Base Editing in Poplar Through an Agrobacterium-Mediated Transformation Method
CRISPR-Cas9 systems have revolutionized genome editing in plants and facilitated gene knockout and functional genomic studies in woody plants, like poplar. However, in tree species, previous studies have only ...
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Article
Boosting plant genome editing with a versatile CRISPR-Combo system
CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided genome engineering in plants. However, these systems are mostly used separately, leaving their combinational potential lar...
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Article
Plant prime editing goes prime
Incorporation of multiple-nucleotide substitution in prime-editing guide RNAs greatly boosts editing efficiency in rice and maize, advancing precise genome editing in plants.
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Article
Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce DNA double-stranded breaks, resulting in mu...
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Article
CRISPR–Act3.0 for highly efficient multiplexed gene activation in plants
RNA-guided CRISPR activation (CRISPRa) systems have been developed in plants. However, the simultaneous activation of multiple genes remains challenging. Here, we develop a highly robust CRISPRa system working...
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Protocol
Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants
Prime editing, a CRISPR-Cas9-derived precise genome editing strategy, was recently developed to introduce targeted indels and all 12 types of point mutations without DNA double-strand breaks or donor DNA. The ...
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Article
PAM-less plant genome editing using a CRISPR–SpRY toolbox
The rapid development of the CRISPR–Cas9, –Cas12a and –Cas12b genome editing systems has greatly fuelled basic and translational plant research1–6. DNA targeting by these Cas nucleases is restricted by their pref...
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Protocol
Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice Protoplasts
CRISPR-Cas9 has revolutionized the field of genome engineering. Base editing, a new genome editing strategy, was recently developed to engineer nucleotide substitutions. DNA base editing systems use a catalyti...
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Article
CRISPR-Cas nucleases and base editors for plant genome editing
Clustered regularly interspaced short palindromic repeats (CRISPR)—CRISPR-associated protein (Cas) and base editors are fundamental tools in plant genome editing. Cas9 from Streptococcus pyogenes (SpCas9), recogn...
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Article
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for genetic manipulation has revolutionized life science over the past few years. CRISPR was first discovered as an adaptiv...
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Article
Open AccessApplication of CRISPR-Cas12a temperature sensitivity for improved genome editing in rice, maize, and Arabidopsis
CRISPR-Cas12a (formerly Cpf1) is an RNA-guided endonuclease with distinct features that have expanded genome editing capabilities. Cas12a-mediated genome editing is temperature sensitive in plants, but a lack ...
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Article
Open AccessAn early mechanical coupling of planktonic bacteria in dilute suspensions
It is generally accepted that planktonic bacteria in dilute suspensions are not mechanically coupled and do not show correlated motion. The mechanical coupling of cells is a trait that develops upon transition...
