19 Result(s)
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Kutubuddin A. Molla
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Article
Open AccessOptimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot
The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail...
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Article
Genome editing for food, nutrition, and health
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Chapter
Genome Editing and Opportunities for Trait Improvement in Pearl Millet
Pearl millet [Cenchrus americanus (L.) Morrone; also known as Pennisetum glaucum], originated 4900 years ago, is a C4 crop with high photosynthetic efficiency and fulfills the food and fodder needs of resource-po...
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Article
Accelerating crop domestication through genome editing for sustainable agriculture
With the establishment of human civilization, wild plant species were domesticated and cultivated at their centres of origin and further disseminated in different parts of the world. While being grown in the w...
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Article
DREB1C: Connecting Dots Between Water- and Nitrogen-Use Efficiency to Climate-Smart Crop Development
Complex traits like water use efficiency (WUE) and nitrogen use efficiency (NUE) are directly associated with crop yield and their stress-tolerant capacity. For develo** climate-smart crops, it is crucial to...
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Article
Protease Inhibitors: An Induced Plant Defense Mechanism Against Herbivores
Plants have evolved with various effective mechanisms to defend themselves against herbivores, and one such mechanism is the production of protease inhibitors that disrupt digestion of the insects. Plant prote...
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Chapter
Genomics and Genome Editing for Crop Improvement
The sustainable growth of agriculture requires continuous efforts towards develo** new plant varieties that can yield more with lesser inputs, specifically, water, fertilizers, and pesticides. Of late the ob...
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Article
Increasing disease resistance in host plants through genome editing
Diseases caused by plant pathogens pose significant challenges to crop yield and quality, thus incurring a serious constraint on the global food supply. Genome editing using the clustered regularly interspaced...
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Article
Open Science: Challenges, Possible Solutions and the Way Forward
Everyone agrees that scientific communication should be free for all. Unfortunately, accessing publications from many reputed journals comes at a high cost—a cost that many researchers and institutions cannot ...
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Article
Comparative transcriptome profiling reveals the basis of differential sheath blight disease response in tolerant and susceptible rice genotypes
Rice sheath blight (ShB) disease, caused by the fungal pathogen Rhizoctonia solani AG1-IA, is one of the devastating diseases and causes severe yield losses all over the world. No completely resistant germplasm i...
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Article
Precise plant genome editing using base editors and prime editors
The development of CRISPR–Cas systems has sparked a genome editing revolution in plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce DNA double-stranded breaks, resulting in mu...
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Protocol
In Vitro Cas9 Cleavage Assay to Check Guide RNA Efficiency
The CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR-associated protein) system has become a popular toolkit for editing genomes of interest in a wide variety of organisms. The Ca...
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Chapter
CRISPR Technologies for Plant Biotechnology Innovation
The invention of CRISPR/Cas technologies and its rapid advancement enables us to modify plant genomes like never before. The Conventional CRISPR/Cas tool has an unparalleled ability to generate targeted mutati...
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Article
Stable quantitative trait locus (QTL) for sheath blight resistance from rice cultivar CR 1014
Sheath blight disease of rice causes substantial crop losses and resistance sources are rare. A moderately resistant genotype CR 1014 was identified and hybridized with highly susceptible genotype Swarna-Sub1....
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Article
Single-nucleotide editing for zebra3 and wsl5 phenotypes in rice using CRISPR/Cas9-mediated adenine base editors
The CRISPR/Cas9-mediated base editing technology can efficiently generate point mutations in the genome without introducing a double-strand break (DSB) or supplying a DNA donor template for homology-directed r...
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Protocol
CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome
Base editing is a recently developed technology that enables us to install point mutation in the genome precisely. The fusion of nucleotide deaminase with Cas9 nickase gives rise to base editors. Like the conv...
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Chapter
Genetic Engineering and Genome Editing Strategies to Enhance Diseases Resistance of Rice Plants: A Review of Progress and Future Prospects
The occurrence of rice diseases threatens food production worldwide. Develo** host resistance is considered as the most efficient and environment-friendly method to reduce yield losses due to the diverse gro...
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Protocol
Wide Horizons of CRISPR-Cas-Derived Technologies for Basic Biology, Agriculture, and Medicine
The discovery and subsequent repurposing of the CRISPR (clustered regularly interspaced short palindromic repeats) and Cas proteins (CRISPR-associated proteins) has revolutionized our ability to manipulate DNA...
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Article
Open AccessDual gene expression cassette is superior than single gene cassette for enhancing sheath blight tolerance in transgenic rice
Sheath blight, caused by the necrotrophic fungal pathogen Rhizoctonia solani, is a serious and destructive disease of the rice. In order to improve sheath blight resistance, we developed three different kinds of ...
