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Article
Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae
Small molecules that exhibit biological effects have been successfully used to study various biological phenomena. 5-Bromodeoxyuridine (BrdU) is a thymidine analog that affects various biological processes, su...
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Article
Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae
5-Bromodeoxyuridine (BrdU) is known to modulate expression of particular genes, and eventually arrest cell division in mammalian and yeast cells. To study a molecular basis for these phenomena, we adopted a ge...
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Article
Bag1 is essential for differentiation and survival of hematopoietic and neuronal cells
Bag1 is a cochaperone for the heat-shock protein Hsp70 that interacts with C-Raf, B-Raf, Akt, Bcl-2, steroid hormone receptors and other proteins. Here we use targeted gene disruption in mice to show that Bag1...
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Article
Heat-shock proteins as regulators of apoptosis
Heat-shock proteins are produced in response to different types of stress conditions making cells resistant to stress-induced cell damage. Under normal conditions, heat-shock proteins play numerous roles in ce...
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Article
Molecular chaperone targeting and regulation by BAG family proteins
Regulated changes in protein conformation can have profound effects on protein function, although routine laboratory methods often fail to detect them. The recently discovered BAG-family proteins may operate a...
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Article
Structural analysis of BAG1 cochaperone and its interactions with Hsc70 heat shock protein
BAG-family proteins share a conserved protein interaction region, called the 'BAG domain', which binds and regulates Hsp70/Hsc70 molecular chaperones. This family of cochaperones functionally regulates signal ...
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Article
Co-expression of bcl-2 and bag-1, apoptosis suppressing genes, prolonged viable culture period of hybridoma and enhanced antibody production
Human bcl-2 and bag-1 DNA were introduced into mouse hybridoma 2E3- O cells and expressed. The expression of bcl-2 in BCMGneo-bcl2 transfectants was confirmed by ELISA and that of bag-1 in pZeo-bag1 was confir...
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Article
BAG-1 accelerates cell motility of human gastric cancer cells
BAG-1 is a Hsp70/Hsc70-binding protein that interacts with Bcl-2, Raf-1, steroid hormone receptors, Siah-1, and hepatocyte growth factor (HGF) receptors, implying multiple functions for the BAG-1 protein. Here...
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Article
Dual radionuclide single-photon emission computed tomography in the prediction of further ischemic risk after acute myocardial infarction
To evaluate whether the findings of dual single-photon emission computed tomography (SPECT) with technetium-99m pyrophosphate (Tc-99m PPi) and thallium-201 were predictive of further cardiac events in their ho...
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Article
Prolonged cell survival enhances peritoneal dissemination of gastric cancer cells
Bcl-2 and a Bcl-2-binding protein BAG-1 function in protection from apoptosis induced by a variety of stimuli. Deregulated expression of Bcl-2 leads to inhibition of apoptosis and is correlated with developmen...
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Article
Erratum: Establishing apoptosis resistant cell lines for improving protein productivity of cell culture
Cytotechnology 23 : 55–59, 1997.
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Article
Expression of multiple apoptosis-regulatory genes in human breast cancer cell lines and primary tumors
The expression of several apoptosis-regulating genes was evaluated in 9 human breast cancer cell lines, 2 immortalized human mammary epithelial lines, 1 normal breast tissue biopsy, and 3 primary breast tumors...
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Article
Anti-apoptotic genes, bag-1 and bcl-2, enabled hybridoma cells to survive under treatment for arresting cell cycle
Hybridoma 2E3-O cells were transfected with bcl-2 alone or with bcl-2 and bag-1 in combination. The bcl-2/bag-1 transfectant survived maintaining viability above 75% for almost 5 days when the cells were treat...
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Article
RT1.P, rat class Ib genes related to mouse TL: evidence that CD1 molecules but not authentic TL antigens are expressed by rat thymus
CD1 and TL were once thought to be genetic homologues because of their thymus-specific expression. We investigated their equivalents in the rat to clarify whether their structure and pattern of expression are co...
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Article
Anti-cell death activity promotes pulmonary metastasis of melanoma cells
Bcl-2 inhibits apoptosis from a variety of stimuli, and a Bcl-2-binding protein BAG-1 also functions in protection from apoptosis in concert with Bcl-2. Here, we provide evidence that prolonged cell survival i...
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Article
Establishing apoptosis resistant cell lines for improving protein productivity of cell culture
The authors established apoptosis resistant COS–1, myeloma, hybridoma, and Friend leukemia cell lines by genetically engineering cells, aiming at more efficient protein production by cell culture. COS–1 cells,...
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Protocol
Protein Interaction Cloning by Far-Western Screening of λ-Phage cDNA Expression Libraries
One of the most powerful ways to gain novel insights into a particular protein’s cellular functions is to identify other proteins with which it specifically interacts. Traditionally, this problem has been appr...
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Chapter
Structure—Function Analysis of Bcl-2 Family Proteins
The Bcl-2 protein blocks a distal step in an evolutionarily conserved pathway for programmed cell death and apoptosis. To gain better understanding of how this protein functions, we have undertaken a structure...
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Chapter
Bcl-2 family proteins and the regulation of programmed cell death in leukemia and lymphoma
Members of the bcl-2 gene family play a central role in regulating the relative sensitivity and resistance of cells to a wide variety of apoptotic stimuli. The first member of this multigene family ,bcl-2, was di...
