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Article
Open AccessTrisomy 12 compromises the mesendodermal differentiation propensity of human pluripotent stem cells
Trisomy 12 is one of the most frequent chromosomal abnormalities in cultured human pluripotent stem cells (hPSCs). Although potential oncogenic properties and augmented cell cycle caused by trisomy 12 have bee...
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Article
Adaptive stimulus selection for consolidation in the hippocampus
Associative memories guide behavioural adaptation by binding together outcome-predictive sensory stimuli1,2. However, in a feature-rich environment, only a subset of stimuli may predict a desired outcome3,4. How ...
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Article
Open AccessVisualization of injectate spread of intercostal nerve block: a cadaveric study
Intercostal nerve block and neurolysis are widely used procedures, but their injectate spread has not been well understood. Previous studies have reported unexpected outcomes (paravertebral or epidural anesthe...
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Article
Anatomical basis for simultaneous block of greater and third occipital nerves, with an ultrasound-guided technique
In some headache disorders, for which the greater occipital nerve block is partly effective, the third occipital nerve is also suggested to be involved. We aimed to establish a simple technique for simultaneou...
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Article
Open AccessSericin in the isolating solution improves the yield of islets isolated from the pancreas
Approximately half of the transplantable pancreatic islet tissue is lost during isolation, including the digestion and purification steps. Modifying the isolation method could increase the yield. This would en...
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Chapter
Regulation of Antigen Presentation by Dendritic Cells and Its Application to Cancer Immunotherapy
Dendritic cells (DCs) are one of the most powerful antigen-presenting cells and play a crucial role in bridging between innate and acquired immunity. Cancer antigens or the long peptides are engulfed by DCs, d...
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Article
Real-time Noninvasive Monitoring of UV Light-induced Cell Death by the Deflection of a Probe Beam
Cell death and its deregulation characterize numerous human diseases. Here, we report on real-time noninvasive monitoring of UV light-induced cell death by the deflection of a probe beam. UV light of 330 – 370...
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Article
Open AccessIdentification of mitogenic factor in rice bran for better mammalian cell culture
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Article
Open AccessUsing Rice Bran Extract (RBE) as Supplement for Mescenchymal Stem Cells (MSCs) in Serum-free Culture
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Article
Open AccessCulture supplement extracted from rice bran for better serum-free culture
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Article
Open AccessRice bran extract (RBE) as supplement for cell culture
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Article
Open AccessCryopreservative solution using rakkyo fructan as cryoprotectant
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Article
Establishment of a mammalian cell line suitable for industrial production of recombinant protein using mutations induced by high-energy beam radiation
Mammalian cells are extensively used for production of biopharmaceuticals. Most cells used in industry have infinite proliferative capacity, which provides a high number of cells and corresponding productivity...
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Article
Rice bran extract affects differentiation of mesenchymal stem cells potency into osteogenic cells
As rice bran contains various nutrients and other proteins of which a part has biological effects on animal cells, we tested the effect of rice bran extract on rat mesenchymal stem cells (rMSCs) obtained from ...
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Chapter and Conference Paper
Change of the Role for JAK/STAT3 in IL-6 Response During Adaptation of Hybridoma Cells to Serum-Free Media
Mammalian cells for production of biologics used in industry are immortal and so they continue multiplying even after they reach required cell number. This excess proliferation consumes nutrients in the medium...
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Chapter and Conference Paper
Study of Growth Factors in Spent Medium for Better Mammalian Cell Culture
Monoclonal antibodies are widely used as anti-cancer drugs, extracorporeal diagnostic agents, etc. They are produced by mammalian cells such as hybridoma and CHO cells. Generally, these cells need growth facto...
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Article
Open AccessMitogenic effect of sericin on mammalian cells
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Chapter and Conference Paper
Fructan as a Novel Effective Factor for Mammalian Cell Culture
Fetal bovine serum (FBS) and growth factors from mammals are widely used in mammalian cell culture. Although effective act for cells, they also have the risk of infection such as bovine spongiform encephalopat...
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Chapter and Conference Paper
Effect of ManNAc and its Derivatives on Glycosylation to Proteins Produced by Mammalian Cell Culture
Various biologics are produced by mammalian cell culture, and for their activities, glycosylation is a pivotal modification. Proteins synthesized in prokaryotes cells such as E. coli were not glycosylated. So pro...
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Chapter and Conference Paper
Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production
Monoclonal antibodies are widely used in molecular biology and in analytical diagnostic tests. The antibody market is increasing every year but to the issue remains as to how to improve the antibody production...
