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Article
An investigation of new commercial samples of Methyl Green and Pyronin Y
New commercial samples of Methyl Green (Gurr Certistain), Pyronine G (Gurr Certistain) and Pyronin Y (Polysciences) have been investigated using spectrophotometry, thin layer chromatography and nuclear magneti...
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Article
Nucleic acid staining with the methyl green-pyronin method
We compared the staining obtained using commercially available pyronin Y samples with that obtained using pure pyronin Y in a standardized methyl green-pyronin procedure. In addition, the importance of the dye...
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Article
Standardized Methyl Green—Pyronin Y procedures using pure dyes
Fully standardized Methyl Green—Pyronin methods are presented. Pure Pyronin Y and purified Methyl Green or Ethyl Green are used either simultaneously in one dye bath or are used as a sequence of Pyronin Y and ...
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Article
Preparation and characterization of methyl green tetrafluoroborate
Commercial methyl green dyes were converted to tetrafluoroborate by means of NaBF4-solution, the compounds thus obtained were analytically pure. It was shown to be possible to distinguish between a “methyl” and a...
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Article
Spectrophotometric characteristics and assay of pure pyronin Y
The spectrophotometric characteristics of analytically pure pyronin Y have been investigated. Addition of metal ions (Fe3+, Zn2+, and Mg2+) and of dextrin were shown not to influence the absorption characteristic...